PE anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
281-2_PE_1_101211
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_1_101211
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown is from gated on myeloid population.
  • 281-2_PE_2_101211
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
142503 25 µg $138
Check Availability


Need larger quantities of this item?
Request Bulk Quote
142504 200 µg $325
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  2. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  3. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  4. Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed
  5. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  6. Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed
  7. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
  8. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  9. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  10. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  11. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  12. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  13. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  14. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  15. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  16. Ribeiro de Almeida C, et al. 2018. Mol Cell. 70:650. PubMed
  17. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  18. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  19. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  20. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  21. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  22. Roy K, et al. 2019. Immunity. 50:616. PubMed
  23. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  24. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  25. Schager AE, et al. 2018. MBio. 9:02379. PubMed
  26. Wang L, et al. 2019. Cancer Lett. 440-441:135. PubMed
  27. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  28. Liao T, et al. 2017. Front Immunol. 8:1334. PubMed
  29. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  30. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  31. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
RRID
AB_10915989 (BioLegend Cat. No. 142503)
AB_10916119 (BioLegend Cat. No. 142504)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD138 (Syndecan-1)

  • PE anti-mouse CD138 (Syndecan-1)

  • APC anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)

  • PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1)

  • Biotin anti-mouse CD138 (Syndecan-1)

  • PE/Cyanine7 anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1)

  • Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1)

  • PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)

  • APC/Cyanine7 anti-mouse CD138 (Syndecan-1)

  • TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1)

  • Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1)

  • TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1)

  • TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1)

  • PE/Cyanine5 anti-mouse CD138 (Syndecan-1)

  • APC/Fire™ 750 anti-mouse CD138 (Syndecan-1)

  • PE/Fire™ 640 anti-mouse CD138 (Syndecan-1)

ProductsHere

Login/Register
Remember me
Forgot your password? Reset Password
Request an Account