PE anti-human/mouse CD49f Antibody

Pricing & Availability
Clone
GoH3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
GoH3_PE_020708
Human peripheral blood lymphocytes stained with GoH3 PE
  • GoH3_PE_020708
    Human peripheral blood lymphocytes stained with GoH3 PE
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313611 25 tests $149
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313612 100 tests $216
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Description

CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Mouse, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Aros CJ, et al. 2020. Cell Stem Cell. 27(3):413-429.e4. PubMed
  2. Aros CJ, et al. 2020. Cell Rep. 30:2055. PubMed
  3. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  4. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  5. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  6. Nanduri L, et al. 2011. Radiother Oncol. 99:367. PubMed
  7. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  8. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  9. Lima-Junior DS, et al. 2021. Cell. . PubMed
  10. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  11. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  12. Morey R, et al. 2021. Front Cell Dev Biol. 9:702046. PubMed
  13. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  14. Zhang D, et al. 2016. Nat Commun. 7:10798. PubMed
  15. Henry G, et al. 2017. Prostate. 77:1344. PubMed
  16. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  17. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  18. Zhao W, et al. 2022. Bioeng Transl Med. 7:e10303. PubMed
  19. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  20. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  21. Kosicki M, et al. 2022. Nat Commun. 13:3422. PubMed
RRID
AB_893374 (BioLegend Cat. No. 313611)
AB_893373 (BioLegend Cat. No. 313612)

Antigen Details

Structure
Integrin family, associates with β1 or β4, 120 kD
Distribution

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Function
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand/Receptor
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
UniProt
View information about CD49f on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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