Brilliant Violet 421™ anti-human/mouse CD49f Antibody

Pricing & Availability
GoH3 (See other available formats)
Regulatory Status
HCDM listed
Other Names
VLA-6 α chain, α6 integrin, integrin α6, ITGA6
Rat IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GoH3) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GoH3) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • GoH3_BV421_CD49f_IHC-P_010318
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of Brilliant Violet 421™ anti-human/mouse CD49f (clone GoH3) antibody (blue) overnight at 4°C. Nuclei were counter-stained with Helix Green (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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313623 25 tests $198
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313624 100 tests $402
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CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Mouse, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Host Species
Mouse mammary tumor cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Moriyama M, et al. 2016. PLoS One. 11:e0164799. PubMed
  2. Yamashiro C, et al. 2020. Nat Protoc. 15:1560. PubMed
  3. Bach K, et al. 2021. Nat Commun. 1502:12. PubMed
  4. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  5. Mok KW et al. 2018. Developmental cell. 48(1):32-48 . PubMed
  6. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  7. Murase Y, et al. 2020. EMBO J. 39:e104929. PubMed
  8. Gell JJ, et al. 2018. Stem Cell Res. 27:46. PubMed
  9. Io S, et al. 2021. Cell Stem Cell. 28(6):1023-1039.e13. PubMed
  10. Lau KX, et al. 2020. Nat Commun. 11:2420. PubMed
  11. Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
  12. Yamashiro C, et al. 2020. Nature Protocols. 362(6412):356-360. PubMed
  13. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  14. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  15. Kojima Y et al. 2017. Cell stem cell. 21(4):517-532 . PubMed
  16. Xiang X, et al. 2022. Nat Commun. 13:463. PubMed
  17. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  18. Sosa E, et al. 2018. Nat Commun. 9:5339. PubMed
  19. Yuan W, et al. 2021. Int J Biol Sci. 17:527. PubMed
  20. Chitiashvili T, et al. 2020. Nat Cell Biol. 22:1436. PubMed
  21. Pandolfi EC, et al. 2022. Cell Rep Med. 3:100782. PubMed
AB_2562243 (BioLegend Cat. No. 313623)
AB_2562244 (BioLegend Cat. No. 313624)

Antigen Details

Integrin family, associates with β1 or β4, 120 kD

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
View information about CD49f on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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