MojoSort™ Human CD14 Nanobeads

Pricing & Availability
Other Names
MojoSort™ Human CD14 Nanobeads, MojoSort™, Human CD14 Positive Selection, CD14 Positive Selection
Ave. Rating
Submit a Review
Product Citations
publications
Mojosort_Human_CD14_Nanobeads_1_082317
PBMCs were prepared to isolate or deplete CD14+ monocytes using the MojoSort™ Human CD14 Nanobeads. Top plot: CD14+ monocytes after isolation. Bottom plot: Cells before isolation. Cells were stained with CD4 (clone RPA-T4) Brilliant Violet 421™ and CD14 (clone HCD14) PE. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_CD14_Nanobeads_1_082317
    PBMCs were prepared to isolate or deplete CD14+ monocytes using the MojoSort™ Human CD14 Nanobeads. Top plot: CD14+ monocytes after isolation. Bottom plot: Cells before isolation. Cells were stained with CD4 (clone RPA-T4) Brilliant Violet 421™ and CD14 (clone HCD14) PE. Dead cells were excluded by 7-AAD.
  • Mojosort_Human_CD14_Nanobeads_2_082317
Cat # Size Price Quantity Avail. Save
480093 20 tests $150
Check Availability


Need larger quantities of this item?
Request Bulk Quote
480094 200 tests $560
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD14+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD14 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480093:

  • 1 vial containing 100 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 1 vial containing 200 µl of Human CD14 Nanobeads

For Cat# 480094:

  • 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
  • 2 vials containing 1 ml of Human CD14 Nanobeads

Product Details

Reactivity
Human
Formulation
Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.
10 µl of Human CD14 Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of human CD14+ cells from peripheral blood mononuclear cells (PBMCs).

 

To find out the dilution to use this product in separation columns please download the CoA.

 

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 08/30/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account