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LEAF™ Purified anti-human TIMP-1 Antibody

Pricing & Availability
Clone
Poly5296 (See other available formats)
Regulatory Status
RUO
Other Names
Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1 (TIMP1), TIMP Metallopeptidase Inhibitor 1, Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
A. Poly5296_LEAF_TIMP1_Neut_060723
LEAF™ purified ant-human TIMP-1 (Poly5296) (purple squares) recovered the activity of recombinant mouse MMP-9 (Cat. No. 755204) in a dose-dependent manner which was inhibited in the presence of recombinant human TIMP-1 (Cat. No. 592404) at 0.33 µg/mL, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range: 0.4 – 4 µg/mL.
  • A. Poly5296_LEAF_TIMP1_Neut_060723
    LEAF™ purified ant-human TIMP-1 (Poly5296) (purple squares) recovered the activity of recombinant mouse MMP-9 (Cat. No. 755204) in a dose-dependent manner which was inhibited in the presence of recombinant human TIMP-1 (Cat. No. 592404) at 0.33 µg/mL, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range: 0.4 – 4 µg/mL.
  • B. Poly5296_LEAF_TIMP1_IHC-P_060723
    IHC staining of LEAF™ purified anti-human TIMP-1 (clone Poly5296) on formalin-fixed paraffin-embedded human pancreas tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) 1.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • Poly5296_LEAF_TIMP1_WB_060723
    Whole cell extracts (15 µg total protein) from HeLa, and NIH/3T3 spiked with or without the indicated amount of recombinant human TIMP-1 protein (Cat. No. 592404) were resolved on a 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL LEAF™ Purified anti-human TIMP-1 (clone Poly5296) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat IgG at a 1:10000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control at a 1:10000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
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529603 100 µg $450
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Description

TIMPs (tissue inhibitors of metalloproteinases) are endogenous inhibitors for MMPs (matrix metalloproteinases). The TIMPs consist of structurally and functionally distinct N- and C-terminal domains, each of which is stabilized by three disulfide bonds. The N-terminal domain binds to the active site of MMPs, inhibiting their proteolytic activities. The bidentate coordination of the zinc ion in the active site of an MMP by the N-terminal α-amino group and carbonyl group of the Cys residue in the "cysteine switch" is a key mechanism of inhibition with MMPs. The TIMP's C-terminal domain is known to bind hemopexin-like domain of pro-MMPs. TIMPs are broad-spectrum inhibitors of MMPs, but there are some differences in specificity among them. Mouse metalloproteinase inhibitor 1, TIMP-1, is more restricted in its inhibitory range than the other three TIMPs, having a relatively low affinity for the membrane-type MMPs, MMP-14, MMP-16, and MMP-24 as well as for MMP-19. Mouse TIMP-1 shares a 72% homology with its human counterpart. It is widely expressed in many mammalian tissues, notably in the reproductive organs. TIMP-1 is able to promote cell proliferation in a wide range of cell types and may also have an anti-apoptotic function. It has anti-angiogenic activity by preventing endothelial cell migration. CD63 has been identified as a receptor for TIMP-1. TIMP-1 binding to CD63 inhibits apoptosis and arrests cell growth. TIMP-1-null mice exhibit a number of alterations in processes associated with reproduction, steroidogenesis and impaired learning and memory. In addition, mice deficient in TIMP-1 exhibit enhanced MMP activity that would contribute to a reduction of atherosclerotic plaque size; nevertheless, it promotes aneurysm formation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human TIMP-1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
IHC-P, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by recovering the activity of recombinant mouse MMP-9 (Cat. No. 755204) that was inhibited in the presence of recombinant human TIMP-1 at 0.33 µg/mL (Cat. No. 592404). ND50 range: 0.4 – 4 μg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2941598 (BioLegend Cat. No. 529603)

Antigen Details

Structure
Monomer
Distribution

It is widely expressed in mammalian tissues, notably in reproductive organs and skin

Function
Angiogenesis, tissue remodeling, cancer metastasis, apoptosis
Interaction
Hematopoietic stem and progenitor cells, CD63 positive cells
Ligand/Receptor
CD63, MMPs, pro-MMP-9
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Death, Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References
  1. Brew K and Nagase H. 2010. Biochim Biophys Acta. 1803:55-71.
  2. Murphy G. 2011. Genome Biol. 12:233
  3. Bourboulia D and Stetler-Stevenson WG. 2010. Semin Cancer Biol. 20:161-8.
  4. Kim YS, et al. 2012. BMB Rep. 45:623-8.
Gene ID
7076 View all products for this Gene ID
UniProt
View information about TIMP-1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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