Keratin 5 Polyclonal Antibody, Purified

Pricing & Availability
Clone
Poly19055 (See other available formats)
Isotype
Rabbit Polyclonal
Purified_Keratin-5_Antibody_IHC_021715
Anti-K5 antibody (clone Poly19055) showing strong cytoplasmic staining of the basal cells of normal human skin epidermis.
  • Purified_Keratin-5_Antibody_IHC_021715
    Anti-K5 antibody (clone Poly19055) showing strong cytoplasmic staining of the basal cells of normal human skin epidermis.
Cat # Size Price Quantity Avail. Save
905501 100 µL $374
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Instructions for Use (pdf)

Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly19055 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 5. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity
Human, Mouse. Rat, Dog, Primate
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by peptide affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:2,000-4,000 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Paraffin-embedded tissues, frozen tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application References

(PubMed link indicates BioLegend citation)
  1. Hübner A, et al. JNK and PTEN cooperatively control the development of invasive adenocarcinoma of the prostate. PNAS, 109: 12046-12051, Jul 2012. [IHC] PubMed
  2. Kierszenbaum AL, Rivkin E, Tres LL. Acroplaxome, an F-actin-keratin-containing plate, anchors the acrosome to the nucleus during shaping of the spermatid head. Mol Biol Cell 14(11):4628-40, 2003. [IHC] PubMed
  3. Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
  4. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  5. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
  6. Liang Y, 2011 Patholog Res Int. 2011:936794. (IHC) PubMed
  7. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
  8. Osmanagic-Myers S, et al.2006. J Cell Biol. 174:557. (WB) PubMed
  9. Kittrell FS, et al. 2011. Breast Cancer Res.13:41. (IF) PubMed
  10. Croyle MJ, et al. Role of epidermal primary cilia in the homeostasis of skin and hair follicles. Development, 138: 1675-1685, May 2011. [IHC, IF] PubMed
  11. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
  12.  
Product Citations
  1. Kierszenbaum A, et al. 2003. Mol Biol Cell. 14:4628-4640. PubMed
  2. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  3. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  4. Hübner A, et al. 2012. Proc Natl Acad Sci U S A. 109:12046-12051. PubMed
  5. Osmanagic-Myers S, et al. 2006. J Cell Biol. 174:557-568. PubMed
  6. Kittrell F, et al. 2011. Breast Cancer Res. 13:R41. PubMed
  7. Croyle M, et al. 2011. Development. 138:1675-1685. PubMed
  8. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  9. Maimets M, et al. 2016. Stem Cell Reports. 6:150-162. PubMed
  10. Johnson D, et al. 2016. PLoS One. 11: 0148851. PubMed
  11. Triplett T, et al. 2016. Proc Natl Acad Sci U S A. 113: 1016 - 1025. PubMed
  12. Tadokoro T, et al. 2016. Development. 143: 764 - 773. PubMed
  13. Wang H, et al. 2016. PLoS One. 11: 0152641. PubMed
  14. Park J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4482-4487. PubMed
  15. Balasooriya G, et al. 2016. Dev Cell. 37: 85-97. PubMed
  16. Linn D, et al. 2016. Cancer Res . 76: 1869-1881. PubMed
  17. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  18. Wang N, et al. 2016. Stem Cell Reports. 6: 668-678. PubMed
  19. Turpin J, et al. 2016. Oncogene. 10.1038/onc.2016.129. PubMed
  20. Papafotiou G, et al. 2016. Nat Commun. 7:11914. PubMed
  21. Wuidart A, et al. 2016. Genes Dev. 30: 1261 - 1277. PubMed
  22. Jullien D, et al. 2016. J Cell Sci. 129: 2673 - 2683. PubMed
  23. Akiyama N, et al. 2016. J Exp Med. 213: 1441 - 1458. PubMed
  24. Wang H, et al. 2016. J Immunol. 197: 141 - 150. PubMed
  25. Adriaens C, et al. 2016. Nat Med. 10.1038/nm.4135. PubMed
  26. Žuklys S, et al. 2016. Nat Immunol. 17:1206-1215. PubMed
  27. Liu S, et al. 2016. PLoS One. 11:e0163232. PubMed
  28. Chiche A, et al. 2016. Oncogene. 10.1038/onc.2016.396. PubMed
  29. Davis F, et al. 2016. Nat Commun. 7:13053. PubMed
  30. Bhagirath D, et al. 2016. PLoS One. 11:e0167064. PubMed
  31. Wu M, et al. 2016. J Biol Chem. 291(49):25749-25760. PubMed
  32. Bohnenpoll T, et al. 2016. J Am Soc Nephrol. 2016080849. PubMed
  33. Yang Z, et al. 2017. Dis Model Mech. 10(1):39-52. PubMed
  34. Song Y, et al. 2017. PLoS One. 12(2):e0171510. PubMed
  35. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
  36. Bohnenpoll T, et al. 2017. PLoS Genet.. 10.1371/journal.pgen.1006951. PubMed
  37. Williams M, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0893-7. PubMed
  38. Li Q, et al. 2018. Neoplasia. 20:233. PubMed
  39. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  40. Lu Y, et al. 2018. Stem Cells. 36:1875. PubMed
  41. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  42. Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed
  43. Dumm RE, et al. 2019. Nat Commun. 10:779. PubMed
  44. Price AJ, et al. 2018. Nat Commun. 9:5284. PubMed
  45. Richards Z, et al. 2019. iScience. 12:304. PubMed
  46. Lee DH, et al. 2019. PLoS One. 14:e0211153. PubMed
  47. Jiao X, et al. 2019. Stem Cell Reports. 12:135. PubMed
  48. Huang M, et al. 2018. J Cell Sci. 131:. PubMed
  49. London NR, et al. 2019. Int Forum Allergy Rhinol. 9:114. PubMed
  50. Wang J, et al. 2018. Cell Rep. 25:464. PubMed
  51. Kullmann FA, et al. 2017. Am J Physiol Renal Physiol. 313:F85. PubMed
  52. Mamo TM, et al. 2017. Hum Mol Genet. 26:3553. PubMed
  53. Shea MP, et al. 2018. Cancer Res. 78:1672. PubMed
  54. Li Q, et al. 2018. J Biol Chem. 293:6434. PubMed
  55. Hollern DP, et al. 2019. Breast Cancer Res Treat. 174:143. PubMed
  56. Kwon OJ, et al. 2019. iScience. 13:328. PubMed
  57. Lecland N, et al. 2019. Life Sci Alliance. 2:2. PubMed
  58. Fang X, et al. 2019. Am J Pathol. 189:1212. PubMed
  59. He Y, et al. 2018. Stem Cells. 36:891. PubMed
  60. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  61. Sépult C, et al. 2019. Oncogene. 38:3521. PubMed
  62. Smith P, et al. 2019. Sci Rep. 9:7079. PubMed
  63. Ramovs V, et al. 2019. Breast Cancer Res. 21:63. PubMed
  64. Bresson L, et al. 2018. Development. 145. PubMed
  65. Dong B, et al. 2018. Development. 145. PubMed
  66. Li Q, et al. 2018. J Biol Chem. 293:10547. PubMed
  67. Ackermann T, et al. 2019. Commun Biol. 2:208. PubMed
  68. Berger A, et al. 2019. J Clin Invest. 130:3924. PubMed
  69. Norum JH, et al. 2019. Int J Cancer. web only. PubMed
  70. Miao Q, et al. 2019. Nat Commun. 10:4042. PubMed
  71. May AJ, et al. 2018. Development. 145. PubMed
  72. Pfefferle AD, et al. 2019. Dis Model Mech. 12:?dmm037192. PubMed
  73. Dedeić Z, et al. 2018. Cell Death Differ. 25:1289. PubMed
  74. Fougner C, et al. 2019. Breast Cancer Res. 21:85. PubMed
  75. Nomeda Girnius et al. 2018. Cell death and differentiation. 25(9):1702-1715 . PubMed
  76. Nomeda Girnius et al. 2018. eLife. 7 pii: e36389. PubMed
  77. Weng PL et al. 2018. Cell reports. 24(6):1464-1470 . PubMed
  78. Ying Yang et al. 2018. Developmental cell. 44(6):752-761 . PubMed
  79. Sreekumar A et al. 2017. Dev Cell. 43(4):436-448 . PubMed
  80. Xiong X et al. 2018. Cell reports. 25(11):3006-3020 . PubMed
  81. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  82. Fu NY et al. 2018. Dev Cell. 47(5):629-644 . PubMed
  83. Girnius N et al. 2017. Cell reports. 21(7):1910-1921 . PubMed
Publication Library
RRID
AB_2565050 (BioLegend Cat. No. 905501)

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 05/11/2017

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account