Keratin 5 Polyclonal Chicken Antibody, Purified

Pricing & Availability
Poly9059 (See other available formats)
Other Names
Keratin, type II cytoskeletal 5, CK-5, 58 kD cytokeratin, type-II keratin Kb5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), krt5
Covance Catalog# SIG-3475
Chicken IgY
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Product Citations
Cat # Size Price Quantity Avail. Save
905901 100 µl $374
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Instructions for Use (pdf)

Product Details

Intended Use
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The polyclonal antibody Poly9059 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Keratin 5. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Human, Mouse
Antibody Type
Host Species
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.
Phosphate-buffered solution + 0.1% sodium azide.
The antibody was purified by IgY affinity chromatography.
1.0 mg/ml
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.


Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:200 -1:1,000 with a biotin based detection system such as biotinylated anti-chicken immunoglobulin and USA Ultra Streptavidin Detection (Cat. No. 929501).

Positive Control tissue: Human skin, mouse skin

Tissue Sections: Paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Sodium Citrate H.I.E.R Retrieval solution (Cat. No. 928502, 928602).
Incubation: 60 minutes at room temperature

Precautions for Use: Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Application References

(PubMed link indicates BioLegend citation)
  1. Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
  2. Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
Product Citations
  1. Coelho P, et al. 2015. Open Bio. 5: 150209. PubMed
  2. Wang N, et al. 2016. Stem Cell Reports. 6: 668-678. PubMed
  3. O’Brien V, et al. 2016. Nat Microbiol. 2:16196. PubMed
  4. Ku A, et al. 2017. eLife. 10.7554/eLife.23242. PubMed
  5. Zhang Y 2018. Cell stem cell. 23:516. PubMed
  6. Yang Y 2018. Developmental cell. 44:752. PubMed
  7. O'Brien VP, et al. 2018. PLoS Pathog. 14:e1007457. PubMed
  8. Wang J, et al. 2018. Cell Rep. 25:464. PubMed
  9. Hoefert JE, et al. 2018. J Cell Biol. 217:2185. PubMed
Publication Library
AB_2565054 (BioLegend Cat. No. 905901)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3852 View all products for this Gene ID
View information about Keratin 5 on

Related FAQs

Does BioLegend manufacture antibodies at GMP grade?

BioLegend antibodies and reagents that carry the IVD or ASR designation are manufactured in a registered GMP facility, while our other products are manufactured under ISO 13485 certification. Both environments provide high quality products. To learn more about our quality standards visit our Quality Control page at:

Go To Top Version: 4    Revision Date: 05/11/2017

Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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