HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody

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Poly4053 (See other available formats)
Goat Polyclonal IgG
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Product Citations
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405306 500 µL $120
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This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Aqueous buffered solution containing a carrier protein and preservatives.
The polyclonal antibody was purified from goat antiserum by mouse immunoglobulin affinity chromatography.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product

ELISA - Quality tested
WB, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:2,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta, C, et al. 2008. J. Immunol. 181:1025. PubMed
Product Citations
  1. Frank N, et al. 2011. Cancer Res. 71:1474. PubMed
  2. Hwang S, et al. 2012. Proc Natl Acad Sci U S A. 110:276. PubMed
  3. Horton N, et al. 2013. PLoS One. 8:e59552. PubMed
  4. Hsing C, et al. 2008. Cytokine. 44:221. PubMed
  5. Yan L, et al. 2014. Clin Vaccine Immunol. 21:1206. PubMed
  6. Copp J, et al. 2014. Proc Natl Acad Sci U S A. 111:13481. PubMed
  7. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  8. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  9. Nishigami Y, et al. 2016. Sci Rep. 6:18964. PubMed
  10. Sertorio M, et al. 2016. J Immunol. 196: 2986-2994. PubMed
  11. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  12. Vrieling M, et al. 2016. Sci Rep. 6:37759. PubMed
  13. Ortega-Prieto AM, et al. 2018. Nat Commun. 9:682. PubMed
  14. Miyamoto M, et al. 2018. Oncol Lett. 15:4005. PubMed
  15. Chen G, et al. 2018. Front Immunol. 9:549. PubMed
  16. Wang C, et al. 2019. PLoS Pathog. 15:e1007938. PubMed
  17. Chung KP, et al. 2019. Nat Commun. 10:3390. PubMed
  18. Chiang CF, et al. 2019. Sci Rep. 9:12448. PubMed
  19. Martinov T, et al. 2019. J Immunol. 203:844. PubMed
  20. Ventura E, et al. 2018. J Cell Sci. 131. PubMed
  21. Omer S et al. 2018. eLife. 7 pii: e36745. PubMed
  22. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  23. Prakash P, et al. 2019. Methods Protoc. 2:48. PubMed
  24. Zhang B, et al. 2020. J Exp Med. 217:e20182365. PubMed
  25. Jin SW, et al. 2019. Cell Rep. 29:1449. PubMed
  26. Parks KR, et al. 2019. Cell Rep. 29:3060. PubMed
  27. Hoffmann D, et al. 2020. Int J Tryptophan Res. 12:1178646919891736. PubMed
AB_315009 (BioLegend Cat. No. 405306)

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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