ELISA Coating Buffer (5X)

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421701 30 mL $19
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Technical data sheet

Product Details

5X concentrated carbonate buffer, pH 9.5.
Storage & Handling
The undiluted solution should be stored between 2°C and 8°C.

ELISA - Quality tested

Recommended Usage

The BioLegend ELISA Coating Buffer(5X) must be diluted to 1X working solution with D.I. water prior to ELISA coating procedures. e.g. Dilute one (1) part ELISA Coating Buffer(5X) to four (4) parts D.I. water. Each lot of BioLegend ELISA Coating Buffer (5X) has been tested with cytokine ELISA capture antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed
  2. Fritz JM, et al. 2014. PLoS One. 9:107473. PubMed
Product Citations
  1. Harland M, et al. 2020. J Neurosci. 1756:40. PubMed
  2. Fritz J, Weaver T 2014. PLoS One. 9:107473. PubMed
  3. Lakshmipriya T, et al. 2017. Int J Biol Macromol. 10.1016/j.ijbiomac.2017.07.115. PubMed
  4. Yoon I, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.07.037. PubMed
  5. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed
  6. Lakshmipriya T, et al. 2016. PLoS One. 11: 0151153. PubMed
  7. Chen X, et al. 2020. bioRxiv. . PubMed
  8. Lim J, et al. 2020. Sci Rep. 10:14897. PubMed

Antigen Details

Gene ID
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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