Direct-Blot™ HRP anti-GST Antibody

Pricing & Availability
Clone
P1A12 (See other available formats)
Other Names
Glutathione-S-transferase
Isotype
Mouse IgG2b, κ
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Product Citations
publications
P1A12_DirectBlotHRP_GST_Antibody_WB_021716
10 µg of recombinant GST protein tagged with HA-tag was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP GST (Clone P1A12). Proteins were visualized using chemiluminescence detection.
  • P1A12_DirectBlotHRP_GST_Antibody_WB_021716
    10 µg of recombinant GST protein tagged with HA-tag was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP GST (Clone P1A12). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
640804 25 µl $85
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640803 100 µl $290
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Description

GST catalyzes the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles, resulting in the detoxification of these compounds.Human GST molecules are classified into four families: alpha, mu, pi, and theta.GST is commonly used as a tag to create fusion proteins in order to facilitate purification and detection of these proteins of interest.

Product Details
Technical data sheet

Product Details

Reactivity
GST tagged protein
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length GST
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicol C, et al. 2011. Biochem Biophys Res Commun. 405:417. PubMed
RRID
AB_2572007 (BioLegend Cat. No. 640803)

Antigen Details

Structure
MW, 22-29 kD
Function
GST catalyzes the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles, resulting in the detoxification of these compounds.
Biology Area
Cell Biology
Antigen References

1. Dejong JL, et al. 1988. Nucleic Acids Res. 16:8541.
2. Alin P, et al. 1985. FEBS Lett. 182:319

Gene ID
56615 View all products for this Gene ID
UniProt
View information about GST on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 04/21/2016

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