Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
400157 25 tests $150
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400158 100 tests $325
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Application References

(PubMed link indicates BioLegend citation)
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Dayang EZ, et al. 2019. Front Immunol. 10:1169. PubMed
  2. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  3. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  4. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  5. Nur Patria Y, et al. 2020. Stem Cell Research. 72.79305556. PubMed
  6. van Stigt Thans T, et al. 2020. Journal of Virology. 93(20):e00719-19.. PubMed
  7. Blackburn M, et al. 2015. J Immunol. 195: 3227 - 3236. PubMed
  8. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  9. Cornelius C et al. 2016. EBioMedicine. 11:58-67 . PubMed
RRID
AB_10897939 (BioLegend Cat. No. 400157)
AB_11150232 (BioLegend Cat. No. 400158)

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 4    Revision Date: 06/11/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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