APC/Fire 750 anti-mouse CD19 Antibody anti-CD19

Pricing & Availability

Clone: 6D5 (See other available formats)
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
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Product Citations: publications

6D5_APCFire750_CD19_Antibody_1_032216 — C57BL/6 splenocytes were stained with CD3 PE and CD19 (clone 6D5) APC/Fire™ 750 (top) or Rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).

Compare all formats See APC/Fire™ 750 spectral data

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Size

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115557

25 µg

$105

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115558

100 µg

$259

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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Application References (PubMed link indicates BioLegend citation)

Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
Olive V, et al. 2013. Elife. 2:822. PubMed
Miyai T, et al. 2014. PNAS. 111:11780. PubMed

Product Citations

Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed
Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed

RRID

AB_2572119 (BioLegend Cat. No. 115557)
AB_2572120 (BioLegend Cat. No. 115558)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

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Brilliant Violet 711™ anti-mouse CD19	6D5	FC
APC/Fire™ 750 anti-mouse CD19	6D5	FC
TotalSeq™-A0093 anti-mouse CD19	6D5	PG
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Ultra-LEAF™ Purified anti-mouse CD19	6D5	FC, CyTOF®, IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody	6D5	FC
Spark YG™ 581 anti-mouse CD19	6D5	FC
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Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/22/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.