PerCP/Cyanine5.5 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_PerCPCyanine55_CD19_Antibody_102318
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • 6D5_PerCPCyanine55_CD19_Antibody_102318
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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115533 25 µg $83
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115534 100 µg $270
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Hajas A, et al. 2013. Hum Immunol. 19:93. PubMed
  2. Li S, et al. 2013. Virology. 443:143. PubMed
  3. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  4. Li S, et al. 2014. J Immunol. 193:306. PubMed
  5. White C, et al. 2015. J Immunol. 194:697. PubMed
  6. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  7. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  8. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  9. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  10. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  11. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  12. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  13. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  14. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  15. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  16. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  17. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  18. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  19. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  20. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  21. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  22. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  23. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  24. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  25. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  26. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  27. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  28. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  29. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  30. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  31. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  32. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  33. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  34. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  35. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  36. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  37. Santiago M, et al. 2010. J Immunol. 185:1114. PubMed
  38. Smith D, et al. 2011. PLoS One. 7:e1002284. PubMed
RRID
AB_2259869 (BioLegend Cat. No. 115533)
AB_2072925 (BioLegend Cat. No. 115534)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC, CyTOF®, IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC, IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F, FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC, IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC, CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC, CyTOF®, IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Go To Top Version: 3    Revision Date: 10/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD19

  • Biotin anti-mouse CD19

  • FITC anti-mouse CD19

  • PE anti-mouse CD19

  • PE/Cyanine5 anti-mouse CD19

  • Purified anti-mouse CD19

  • PE/Cyanine7 anti-mouse CD19

  • Alexa Fluor® 488 anti-mouse CD19

  • Alexa Fluor® 647 anti-mouse CD19

  • Pacific Blue™ anti-mouse CD19

  • Alexa Fluor® 700 anti-mouse CD19

  • APC/Cyanine7 anti-mouse CD19

  • PerCP anti-mouse CD19

  • PerCP/Cyanine5.5 anti-mouse CD19

  • Alexa Fluor® 594 anti-mouse CD19

  • Brilliant Violet 421™ anti-mouse CD19

  • Brilliant Violet 570™ anti-mouse CD19

  • Brilliant Violet 605™ anti-mouse CD19

  • Brilliant Violet 650™ anti-mouse CD19

  • Brilliant Violet 785™ anti-mouse CD19

  • Brilliant Violet 510™ anti-mouse CD19

  • Purified anti-mouse CD19 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD19

  • Brilliant Violet 711™ anti-mouse CD19

  • APC/Fire™ 750 anti-mouse CD19

  • TotalSeq™-A0093 anti-mouse CD19

  • Brilliant Violet 750™ anti-mouse CD19

  • TotalSeq™-B0093 anti-mouse CD19

  • Spark Blue™ 550 anti-mouse CD19

  • Spark NIR™ 685 anti-mouse CD19

  • TotalSeq™-C0093 anti-mouse CD19

  • Ultra-LEAF™ Purified anti-mouse CD19

  • PE/Fire™ 640 anti-mouse CD19 Antibody

  • Spark YG™ 581 anti-mouse CD19

  • APC/Fire™ 810 anti-mouse CD19

  • Spark YG™ 570 anti-mouse CD19

  • Spark Blue™ 574 anti-mouse CD19 Antibody

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