APC anti-human CD62L Antibody

Pricing & Availability
Clone
DREG-56 (See other available formats)
Workshop
V S056
Other Names
L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
DREG-56_APC_070207
Human peripheral blood lymphocytes stained with DREG-56 APC
  • DREG-56_APC_070207
    Human peripheral blood lymphocytes stained with DREG-56 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
304809 25 tests $125
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304810 100 tests $265
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Description

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee, Cattle (Bovine, Cow)
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 304812).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block)
  3. Jutila M, et al. 2002. J. Immunol. 169:1768. (WB)
  4. Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed
  5. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB)
  10. Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed
  11. Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed
  12. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations
  1. Jin C, et al. 2012. J Biol Chem. 287:35922. PubMed
  2. Ryba M, et al. 2011. Cytokine. 55:353. PubMed
  3. Trinité B, et al. 2014. PLoS One. 9:110719. PubMed
  4. Hünniger K, et al. 2015. J Immunol. 194:1199. PubMed
  5. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  6. Lieberman L, et al. 2016. Neurology. 86: 375 - 381. PubMed
  7. Nakajima-Adachi H, et al. 2017. PLoS One. 12(2):e0172795. PubMed
  8. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  9. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  10. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
Publication Library
RRID
AB_314469 (BioLegend Cat. No. 304809)
AB_314470 (BioLegend Cat. No. 304810)

Antigen Details

Structure
Selectin, single chain glycoprotein, 74-95 kD
Distribution

Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes

Function
Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor
CD34, GlyCAM, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, NK cells, T cells, Thymocytes, Neutrophils, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
2. Kishimoto T, et al. 1991. Blood 78:805.

Gene ID
6402 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

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Go To Top Version: 2    Revision Date: 05/21/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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