Alexa Fluor® 647 anti-mouse CD49b Antibody

Pricing & Availability
Clone
HMα2 (See other available formats)
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMalpha2_Alx647_100507
C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 647 and DX5 PE
  • HMalpha2_Alx647_100507
    C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 647 and DX5 PE
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
103511 25 µg $85
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Description

CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse colon carcinoma cell line Colon26
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
  4. Zhang Z, et al. 2008. Blood 111:1980. (IF)
  5. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  6. Schwartz C, et al. 2014. PNAS. 111:5169. PubMed
Product Citations
  1. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  2. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  3. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  4. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  5. Tao L, et al. 2017. Nat Commun. 8:14431. PubMed
  6. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  7. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_528830 (BioLegend Cat. No. 103511)

Antigen Details

Structure
Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution
NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
Function
Adhesion, lymphocyte activation
Ligand Receptor
Collagen, laminin, fibronectin
Cell Type
NK cells, T cells, NKT cells, Epithelial cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.

Gene ID
16398 View all products for this Gene ID
UniProt
View information about CD49b on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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