- HMα2 (See other available formats)
- Other Names
- α2 integrin, VLA-2 α chain, Integrin α2 chain, ITGA2, HMa2
- Armenian Hamster IgG
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- Product Citations
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CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Mouse colon carcinoma cell line Colon26
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IF - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence on frozen sections4, and blocking of cell adhesion1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays.
(PubMed link indicates BioLegend citation)
- Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (Block, IP)
- Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
- Arase H, et al. 2001. J. Immunol. 167:1141. (Block)
- Zhang Z, et al. 2008. Blood 111:1980. (IF)
- Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
- Schwartz C, et al. 2014. PNAS. 111:5169. PubMed
- Product Citations
AB_528830 (BioLegend Cat. No. 103511)
- Integrin family, associates with CD29 (β1 integrin), 150 kD
- NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
- Adhesion, lymphocyte activation
- Ligand Receptor
- Collagen, laminin, fibronectin
- Cell Type
- NK cells, T cells, NKT cells, Epithelial cells, Lymphocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Sasaki K, et al. 2003. Int. Immunol. 15:701.
3. Inoue O, et al. 2003. J. Cell Biol. 160:769.
- Gene ID
- 16398 View all products for this Gene ID
- View information about CD49b on UniProt.org
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