Alexa Fluor® 488 Streptavidin

Pricing & Availability
Regulatory Status
Other Names
Streptavidin-Alexa Fluor® 488, Streptavidin-AF488
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Product Citations
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 488.
  • Strepavidin_A488_Antibody_FC_013114
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or biotinylated mouse IgG1 isotype control (open histogram), followed by SAV-Alexa Fluor® 488.
See Alexa Fluor® 488 spectral data
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405235 100 µg $206
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Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 488 is useful for detecting biotinylated antibodies. The excitation of Alexa Fluor® 488 by 488 nm laser light induces a light emission maximum of 519 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Streptavidin was conjugated with Alexa Fluor® 488 under optimal conditions.
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 488 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

ICFC - Verified

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 488 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-Alexa Fluor® 488 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 488 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed
  3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
  4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
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  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed.
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
Product Citations
  1. Tran JR, et al. 2021. J Cell Biol. 220: . PubMed
  2. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  3. Han Y, et al. 2020. Vaccines (Basel). 8:00. PubMed
  4. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  5. Aneas I, et al. 2021. Nat Commun. 12:6115. PubMed
  6. Tomić S, et al. 2016. Sci Rep. 6:31618. PubMed
  7. Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed
  8. Tomić S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.08.040. PubMed
  9. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  10. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  11. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  12. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  13. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  14. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  15. Lederer K, et al. 2022. Cell. . PubMed
  16. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  17. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  18. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  19. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  20. Tichy ED, et al. 2021. Cell Rep. 35:109098. PubMed

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Go To Top Version: 5    Revision Date: 06/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.


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