Optimized Multicolor Flow Cytometry Panels

 

Building a successful multicolor flow cytometry panel often requires extensive optimization – fluorophore/marker selection, antibody titrations, and buffer selection, among other factors.

 

Here, we have compiled multicolor flow cytometry panels and the associated protocols that have been designed and optimized by our expert scientists. This helps remove some of the guesswork from panel and experimental design, allowing you to advance toward new discoveries more quickly.

 

Each panel set is assigned a panel bundle promotion code that corresponds to all associated reagents necessary to replicate the experiment (e.g. fluorophore-conjugated antibodies, buffers, and other solutions). For ordering information, contact our sales team.

Click below to find a multicolor panel

Click the plus next to each panel to display a quick preview.

Marker Fluorophore
CCR7 (CD197) BV421™
CD20 Pacific Blue™
IgM BV510™
CD3 BV570™
CD16 BV605™
CD28 BV650™
CD38 BV711™
CD56 BV750™
PD-1 BV785™
Marker Fluorophore
CD45RA Alexa Fluor® 488
CD14 Spark Blue™ 550
CD4 Spark Blue™ 574
CD8 PerCP/Cyanine5.5
Marker Fluorophore
TCR γδ PE
IgD Spark YG™ 581
CD141 PE/Dazzle™ 594
CD25 PE/Fire™ 700
CD11c PE/Cyanine7
HLA-DR PE/Fire™ 810
Marker Fluorophore
CD127 APC
CD1c Alexa Fluor® 647
CD19 Spark NIR™ 685
CD123 Alexa Fluor® 700
Viability Zombie NIR™
CD45 APC/Fire™ 750
CD27 APC/Fire™ 810

Marker Fluorophore
CCR7 (CD197) BV421™
CD20 Pacific Blue™
IgM BV510™
CD3 BV570™
CD16 BV605™
CD28 BV650™
CD38 BV711™
CD56 BV750™
PD-1 BV785™
Marker Fluorophore
CD45RA Alexa Fluor® 488
CD14 Spark Blue™ 550
CD4 Spark Blue™ 574
CD8 PerCP/Cyanine5.5
Marker Fluorophore
TCR γδ PE
IgD Spark YG™ 581
CD141 PE/Dazzle™ 594
CD25 PE/Fire™ 700
CD11c PE/Cyanine7
HLA-DR PE/Fire™ 810
Marker Fluorophore
CD127 APC
CD1c Alexa Fluor® 647
CD19 Spark NIR™ 685
CD123 Alexa Fluor® 700
Viability Zombie NIR™
CD45 APC/Fire™ 750
CD27 APC/Fire™ 810

Marker Fluorophore
CCR7 (CD197) BV421™
CD20 Pacific Blue™
IgM BV510™
CD3 BV570™
CD16 BV605™
CD28 BV650™
CD38 BV711™
CD56 BV750™
PD-1 BV785™
Marker Fluorophore
CD45RA Alexa Fluor® 488
CD14 Spark Blue™ 550
CD4 Spark Blue™ 574
CD8 PerCP/Cyanine5.5
Marker Fluorophore
TCR γδ PE
IgD Spark YG™ 581
CD141 PE/Dazzle™ 594
CD25 PE/Fire™ 700
CD11c PE/Cyanine7
HLA-DR PE/Fire™ 810
Marker Fluorophore
CD127 APC
CD1c Alexa Fluor® 647
CD19 Spark NIR™ 685
CD123 Alexa Fluor® 700
Viability Zombie NIR™
CD45 APC/Fire™ 750
CD27 APC/Fire™ 810

Marker Fluorochrome
CCR7 (CD197) BV421™
CD28 Pacific Blue™
CD3 BV510™
CD127 BV570™
CTLA4 BV605™
Tim-3 BV650™
CD73 BV711™
CXCR5 (CD185) BV750™
CD57 BV785™
Marker Fluorochrome
TCR Vα7.2 FITC
CD8 Spark Blue™ 550
CD45 Spark Blue™ 574
CD244 PerCP/Cyanine5.5
Marker Fluorochrome
PD-1 (CD279) PE
CD4 Spark YG™ 581
CD95 PE/Dazzle™ 594
TIGIT PE/Fire™ 640
CD25 PE/Cyanine5
TCR γδ PE/Fire™ 700
CD39 PE/Cyanine7
KLRG1 PE/Fire™ 810
Marker Fluorochrome
LAG3 APC
CD45RA Spark NIR™ 685
CD19, CD14 Alexa Fluor® 700
Viability Zombie NIR™
CD27 APC/Fire™ 750
HLA-DR APC/Fire™ 810

Marker Fluorophore
CCR7 (CD197) BV421™
CD28 Pacific Blue™
CD3 BV510™
CD127 BV570™
CXCR3 (CD183) BV605™
CCR6 (CD196) BV650™
ICOS (CD278) BV711™
CXCR5 (CD185) BV750™
CD161 BV785™
Marker Fluorophore
TCR Vα7.2 FITC
CD8 Spark Blue™ 550
CD45 Spark Blue™ 574
CCR5 (CD195) PerCP/Cyanine5.5
Marker Fluorophore
PD-1 (CD279) PE
CD4 Spark YG™ 581
CD95 PE/Dazzle™ 594
CD38 PE/Fire™ 640
CD25 PE/Cyanine5
TCR γδ PE/Fire™ 700
CD39 PE/Cyanine7
CCR4 (CD194) PE/Fire™ 810
Marker Fluorophore
CCR10 APC
CD45RA Spark NIR™ 685
CD19, CD14 Alexa Fluor® 700
Viability Zombie NIR™
CD27 APC/Fire™ 750
HLA-DR APC/Fire™ 810

Notes:

  • Users’ experimental results may vary from what is shown as representative data due to numerous factors including, but not limited to: instrument setup and other auxiliary reagents used, inherent variability in biological samples, and user technique.
  • Using a flow cytometer that is compatible with selected panel is critical for success. Prior to ordering, we urge users to check available flow cytometers and their specification to ensure it is compatible with the panel.
  • Please contact our technical services team if you have any questions or require assistance on any of the above points.
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