Optimized Multicolor Flow Cytometry Panels

 

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Human General Phenotyping (26c)

 

A 26-color panel to examine major immune cell subsets from human peripheral blood. Panel optimization was performed using a 5-laser Cytek Aurora.

 

  • Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
  • Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
  • Product Name: Human General Phenotyping (26c)

 

Marker, Fluorophore, and Clone List:

 

Product Clone    
Brilliant Violet 421™ anti-human CD197 (CCR7) G043H7 View Product
Pacific Blue™ anti-human CD20 2H7 View Product
Brilliant Violet 510™ anti-human IgM MHM-88 View Product
Brilliant Violet 570™ anti-human CD3 UCHT1 View Product
Brilliant Violet 605™ anti-human CD16 3G8 View Product
Brilliant Violet 650™ anti-human CD28 CD28.2 View Product
Brilliant Violet 711™ anti-human CD38 HIT2 View Product
Brilliant Violet 750™ anti-human CD56 (NCAM) 5.1H11 View Product
Brilliant Violet 785™ anti-human CD279 (PD-1) EH12.2H7 View Product
Alexa Fluor® 488 anti-human CD45RA HI100 View Product
Spark Blue™ 550 anti-human CD14 63D3 View Product
Spark Blue™ 574 anti-human CD4 SK3 View Product
PerCP/Cyanine5.5 anti-human CD8 SK1 View Product
PE anti-human TCR γ/δ B1 View Product
Spark YG™ 581 anti-human IgD W18340F View Product
PE/Dazzle™ 594 anti-human CD141 (Thrombomodulin) M80 View Product
PE/Fire™ 700 anti-human CD25 M-A251 View Product
PE/Cyanine7 anti-human CD11c 3.9 View Product
PE/Fire™ 810 anti-human HLA-DR L243 View Product
APC anti-human CD127 (IL-7Rα) A019D5 View Product
Alexa Fluor® 647 anti-human CD1c L161 View Product
Spark NIR™ 685 anti-human CD19 HIB19 View Product
Alexa Fluor® 700 anti-human CD123 6H6 View Product
Zombie NIR™ Fixable Viability Kit Viability View Product
APC/Fire™ 750 anti-human CD45 2D1 View Product
APC/Fire™ 810 anti-human CD27 O323 View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)   View Product
Cell Staining Buffer   View Product
True-Stain Monocyte Blocker™   View Product
Tandem Stabilizer   View Product
Compensation Beads   View Product

 

Representative data:

 

Human PBMCs were stained with the Human General Phenotyping (26-color) panel and acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora. Cells were gated for 1) T cells, 2) Basophils, or 3) Monocytes, NK cells, dendritic cells, B cells, and ILCs.

 

Notes:

  • Users’ experimental results may vary from what is shown as representative data due to numerous factors including, but not limited to: instrument setup and other auxiliary reagents used, inherent variability in biological samples, and user technique.
  • Using a flow cytometer that is compatible with selected panel is critical for success. Prior to ordering, we urge users to check available flow cytometers and their specification to ensure it is compatible with the panel.
  • Please contact our technical services team if you have any questions or require assistance on any of the above points.
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