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HRP anti-Tau Phospho (Ser262) Antibody

Pricing & Availability
Clone
A15091A (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule associated protein tau
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15091A_HRP_Tau-Phospho_Antibody_041218_merged
IHC staining of HRP conjugated anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer's disease (right panel) human brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
  • A15091A_HRP_Tau-Phospho_Antibody_041218_merged
    IHC staining of HRP conjugated anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer's disease (right panel) human brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyperphosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Tau Phospho (Ser262) peptide conjugated to KLH.
Purity
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins
Antigen References

1. Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35. PubMed.
2. Seubert P, et al. 1995. J Biol Chem 270(32):18917-22. PubMed.
3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.

Gene ID
4137 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Tau Phospho Ser262
Specificity Alt (DOES NOT SHOW ON TDS):
Tau Phospho (Ser262)
App Abbreviation (DOES NOT SHOW ON TDS):
IHC-P
UniProt
View information about Tau Phospho Ser262 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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