Purified anti-Tau Phospho (Ser262) Antibody

Pricing & Availability
Clone
A15091A (See other available formats)
Other Names
Microtubule associated protein tau
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_A15091A_PURE_Tau_Phospho_Ab_IHC_021918
IHC staining of anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 10 µg/ml for 2 hours at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The antibody specificity was confirmed by inhibiting its staining by pre-incubating the mAb with a 10-fold molar excess of pS262 tau peptide-BSA. In contrast, incubation with a 10-fold molar excess of a pT181 Tau peptide-BSA did not alter the antibody’s activity.
  • 1_A15091A_PURE_Tau_Phospho_Ab_IHC_021918
    IHC staining of anti-Tau Phospho (Ser262) antibody (clone A15091A) on formalin-fixed paraffin-embedded Alzheimer’s brain tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 10 µg/ml for 2 hours at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The antibody specificity was confirmed by inhibiting its staining by pre-incubating the mAb with a 10-fold molar excess of pS262 tau peptide-BSA. In contrast, incubation with a 10-fold molar excess of a pT181 Tau peptide-BSA did not alter the antibody’s activity.
  • 2_A15091A_PURE_Tau_Phospho_Ab_ELISA_021918
    Direct ELISA of purified anti-Tau Phospho (Ser262) antibody (clone A15091A) binding to plate-immobilized pS262 Tau peptide conjugated to BSA. The antibody did not react with the same BSA-conjugated peptide that was not phosphorylated or whose sequence was scrambled. ELISA was performed by coating wells with 150 ng of each recombinant Tau protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
Cat # Size Price Quantity Avail. Save
849501 25 µg $100
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849502 100 µg $255
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Tau Phospho (Ser262) peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Direct ELISA - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For Direct ELISA, the suggested use of this reagent is 0.05 - 1.0 per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687294 (BioLegend Cat. No. 849501)
AB_2687295 (BioLegend Cat. No. 849502)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.

Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins
Antigen References

1. Augustinack JC, et al. 2002. Acta Neuropath. 103(1):26-35. PubMed.
2. Seubert P, et al. 1995. J Biol Chem 270(32):18917-22. PubMed.
3. Dong Y, et al. 2012. PLoS One 7(6):e39386. PubMed.

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho (Ser262) on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/21/2018

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