- MR1 (See other available formats)
- Regulatory Status
- Other Names
- CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Activated mouse Th1 clone D1.6
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).
- Application References
- Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
- Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
- Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
- Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
- Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Product Citations
AB_313270 (BioLegend Cat. No. 106505)
AB_313271 (BioLegend Cat. No. 106506)
- TNF superfamily, 39 kD
Activated CD4+ T cells
- T-B cell costimulation
- Cell Type
- T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
- Gene ID
- 21947 View all products for this Gene ID
- View information about CD154 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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