Ultra-LEAF™ Purified anti-mouse CD20 Antibody

Pricing & Availability
Clone
SA271G2 (See other available formats)
Regulatory Status
RUO
Other Names
Ms4a1, Ly-44
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA271G2_PURE_ULEAF_CD20_Antibody_3_012122
C57BL/6 mice were injected intravenously with 250 μg of Ultra-LEAF™ Purified anti-mouse CD20 (clone SA271G2) (top panel) or Ultra-LEAF™ purified rat IgG2b, isotype control (bottom panel). At day 7, the mice were sacrificed, spleens were collected, sectioned and stained for microscopy analysis. CD19 is shown in red, CD3 in cyan, CD169 in green, CD11b in yellow, and F4/80 in blue.
  • SA271G2_PURE_ULEAF_CD20_Antibody_3_012122
    C57BL/6 mice were injected intravenously with 250 μg of Ultra-LEAF™ Purified anti-mouse CD20 (clone SA271G2) (top panel) or Ultra-LEAF™ purified rat IgG2b, isotype control (bottom panel). At day 7, the mice were sacrificed, spleens were collected, sectioned and stained for microscopy analysis. CD19 is shown in red, CD3 in cyan, CD169 in green, CD11b in yellow, and F4/80 in blue.
  • SA271G2_PURE_ULEAF_CD20_Antibody_4_012122
  • SA271G2_ULEAF_CD20_Antibody_1_070616
    C57BL/6J mice were injected i.v. with 250 µg of Ultra-LEAF™ purified anti-mouse CD20 (clone SA271G2) (top) or Ultra-LEAF™ purified rat IgG2b, κ isotype control. At day 7 the mice were bled and the samples were stained with anti-mouse CD19 APC and anti-mouse CD45 Brilliant Violet 421™. Data shown is representative of 4 mice per group.
  • SA271G2_ULEAF_CD20_Antibody_2_070616
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152104 1 mg £102
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152115 5 mg £369
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152116 25 mg £964
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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD20 transfected cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Clone SA271G2 has been validated for in vivo depletion of mouse B cells. Learn more...

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References

(PubMed link indicates BioLegend citation)
  1. Bongard, A, et al. 2019. PLoS Pathog. 15(9):e1008043. (Depletion) Pubmed
Product Citations
  1. Chen YQ, et al. 2019. J Immunother Cancer. 0.415277778. PubMed
  2. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  3. Sodir NM, et al. 2020. Cancer Discov. 0.825. PubMed
RRID
AB_2629619 (BioLegend Cat. No. 152104)
AB_2629619 (BioLegend Cat. No. 152115)
AB_2629619 (BioLegend Cat. No. 152116)

Antigen Details

Structure
Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution

B cells and a subset of T cells

Function
Regulates B-cell activation and proliferation
Interaction
Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3.Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.

Gene ID
12482 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 07/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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