Recombinant Human FGF-9 (Animal-Free)

Pricing & Availability
Other Names
Fibroblast Growth Factor-9, Glia-activating factor (GAF), Heparin-binding growth factor 9 (HBGF-9)
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710604 20 µg £144
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Description

FGF-9 is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family. FGF-9 was first identified as a secretory protein from the human glioma cell line NMC-G1 and is reported to be widely expressed in rat brain. In the central nervous system, FGF-9 acts as an autocrine and a paracrine growth factor that is required for the development, growth, and survival of neuronal cells. During prenatal stages, FGF-9 expression is crucial for normal lung and airway development. FGF-9 null mutant mice show the phenotype of severe lung hypoplasia, which results in perinatal death. FGF-9 is key in stimulating cell proliferation after arterial, skeletal, and hepatic injuries. FGF-9 is also involved in tumorgenesis, enhancing transformation and progression of several malignant diseases, including prostate, lung, and colon cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human FGF-9, amino acids (Pro3 - Ser208) (Accession# NP_002001.1), was expressed in E. coli.
Molecular Mass
The 206 amino acid recombinant protein has a predicted molecular mass of approximately 23.2 kD. The predicted N-terminal amino acid is Pro.
Purity
>95%, as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Concentration
This product is bottled at the concentration indicated on the vial.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate thymidine uptake by BaF3 cells expressing FGF receptors in a dose dependent manner.
Application

Bioassay

Application Notes

This product is reactive with human and mouse.

Antigen Details

Structure
Growth factor
Distribution

FGF-9 is a secreted protein, widely expressed in central nervous system. FGF-9 is expressed in lung mesothelium and epithelium during organogenesis.

Function
FGF-9 is a growth factor and is involved in embryonic development, cell growth, cell differentiation, cell migration, and carcinogenesis. FGF-9 is required for the development of glial cells in nervous system.
Interaction
FGF-9 targets glial cells in nervous system.
Ligand/Receptor
FGFR1-IIIc, FGFR2-IIIc, FGFR3-IIIb, FGFR3-IIIc, and FGFR4.
Bioactivity
Human FGF-9 is able to induce proliferation of BaF3 cells expression FGFR.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Reuss B, et al. 2003. Cell Tissue Res. 313:139.
2. Tsai SJ, et al. 2002. Endocrinology. 143:2715.
3. White AC, et al. 2006. Development. 133:1507.
4. Workalemahu G, 2004. J. Leukoc. Biol. 75:657.
5. Teishima J, 2014. Anticancer Res. 34:695.
6. Behr B, 2010. Proc. Natl. Acad. Sci. USA. 107:11853.

Gene ID
2254 View all products for this Gene ID
UniProt
View information about FGF-9 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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