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Western-Ready™ ECL Substrate Plus Kit

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Western-Ready_ECL_Substrate_Plus_Kit_1_081020
Western blot analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: The indicated amounts of whole cell extracts from HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Lamin A/C antibody (clone WL4G10) (Cat. No. 600002) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution, followed by incubation with Western-Ready™ ECL Substrate Plus Kit or Western-Ready™ ECL Substrate Premium Kit.
  • Western-Ready_ECL_Substrate_Plus_Kit_1_081020
    Western blot analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: The indicated amounts of whole cell extracts from HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-Lamin A/C antibody (clone WL4G10) (Cat. No. 600002) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution, followed by incubation with Western-Ready™ ECL Substrate Plus Kit or Western-Ready™ ECL Substrate Premium Kit.
  • Western-Ready_ECL_Substrate_Plus_Kit_2_081020
    Slot-blot analysis of Western-Ready™ ECL Substrate Plus Kit: The indicated amount of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was immobilized on a nitrocellulose membrane and incubated with Western-Ready™ ECL Substrate Plus Kit prior to chemiluminscent detection at the indicated time points.
  • Western-Ready_ECL_Substrate_Plus_Kit_3_081020
    ELISA analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: The indicated amounts of purified anti-SSRP1 antibody (clone 10D1) (Cat. No. 609702) were captured by anti-mouse FC -coated 96-well plates for 1 hour at room temperature. Bound antibody was detected with HRP goat anti-mouse IgG antibody (Cat. No. 405306) using either Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) (blue triangles) or Western-Ready™ ECL Substrate Plus Kit (red circles). Shown values represent the average signal of three technical replicates after background subtraction.
  • Western-Ready_ECL_Substrate_Plus_Kit_4_082020
    ELISA analysis using Western-Ready™ ECL Substrate Plus and Premium Kits: Bar graph representation of low picogram to high femtogram values shown in panel C.
Cat # Size Price Quantity Check Availability Save
426315 20 mL £41
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426316 200 mL £121
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426317 500 mL £201
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Description

Western-Ready™ ECL Substrate Plus Kit is a sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects picogram amounts of antigen with stable light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Proprietary
Preparation
To prepare working solution, mix equal volumes of Western-Ready™ ECL Substrate Plus Solution A with Western-Ready™ ECL Substrate Plus Solution B. Add 0.1 mL of working solution per cm2 of membrane, and uniformly distribute over the entire membrane surface.
Storage & Handling
Ambient room temperature
Application

WB - Quality tested
ELISA - Verified
Application Notes

BioLegend offers two luminol-based enhanced chemiluminescent substrate solution packages for the detection of horseradish peroxidase on immunoblots and ELISA plates: Western-Ready™ ECL Substrate Plus Kit and Western-Ready™ ECL Substrate Premium Kit. The Western-Ready™ ECL Substrate Plus Kit detects mid- to low- picogram levels of antigen, while the Western-Ready™ ECL Substrate Premium Kit detects high femtogram levels of antigen and is suitable for applications where more sensitive detection is required.

Additional Product Notes

20 mL size (Cat. No. 426315) contains 10 mL each of solution A and solution B.

200 mL size (Cat. No. 426316) contains 100 mL each of solution A and solution B.

500 mL size (Cat. No. 426317) contains 250 mL each of solution A and solution B.

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 08/10/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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