Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-selectin) Antibody

Pricing & Availability
Clone
RME-1/CD62E (See other available formats)
Regulatory Status
RUO
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RME-1-CD62E_ULEAF_CD62E_Antibody_111219
Mouse brain endothelial cell line bEND.3 cells were stimulated with TNF-α for five hours. Then the cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes, subsequently stained with 5 µg/ml of Ultra-LEAF™ purified anti-mouse/rat CD62E (clone RME-1/CD62E) (red) in blocking buffer overnight at 4°C. Then DyLight™ 594 Goat anti-mouse IgG was added for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
  • RME-1-CD62E_ULEAF_CD62E_Antibody_111219
    Mouse brain endothelial cell line bEND.3 cells were stimulated with TNF-α for five hours. Then the cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes, subsequently stained with 5 µg/ml of Ultra-LEAF™ purified anti-mouse/rat CD62E (clone RME-1/CD62E) (red) in blocking buffer overnight at 4°C. Then DyLight™ 594 Goat anti-mouse IgG was added for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
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148803 500 µg £238
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat E-selectin transfected chinese hamster ovary cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

IHC-F - Quality tested
ICC, WB, IP, ELISA, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5, Western blotting1, immunoprecipitation1, ELISA7, and blocking1,2,3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 148803).

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Hybridoma 16:249. (Block, WB, IP)
  2. Walter UM, et al. 1997. Immunology 92:290. (Block, Rat Reactivity)
  3. Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block, Mouse Reactivity)
  4. Bernardes-Silva M, et al. 2001. J. Cereb. Blood Flow Metab. 21:1115. (IHC)
  5. Alvarez A, et al. 2004. Blood 104:402. (IHC, Rat Reactivity)
  6. Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
  7. Walter UM, et al. 1997. in Hybridoma 16:355. (ELISA)
RRID
AB_2565818 (BioLegend Cat. No. 148803)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells

Function
Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
  2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
  3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
  4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
  5. Walz G, et al. 1990. Science 250:1132
Gene ID
20351 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD62E Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse/rat CD62E (E-selectin) RME-1/CD62E IHC-F, ICC, WB, IP, ELISA, Block
Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-selectin) RME-1/CD62E IHC-F, ICC, WB, IP, ELISA, Block
Go To Top Version: 2    Revision Date: 11/12/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse/rat CD62E (E-selectin)

  • Ultra-LEAF™ Purified anti-mouse/rat CD62E (E-selectin)

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