Ultra-LEAF™ Purified anti-mouse CD66a (CEACAM1a) Antibody

Pricing & Availability
Clone
MAb-CC1 (See other available formats)
Other Names
CEACAM1a, Bgp
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_Mab-CC1_PE_071009
Balb/c mouse splenocytes stained with MAb-CC1 PE (lower panel) or mouse IgG1 PE isotype control (upper panel) and CD45R/B220 (RA3-6B2) APC.
  • A_Mab-CC1_PE_071009
    Balb/c mouse splenocytes stained with MAb-CC1 PE (lower panel) or mouse IgG1 PE isotype control (upper panel) and CD45R/B220 (RA3-6B2) APC.
  • B_Mab-CC1_PE_071009
Cat # Size Price Quantity Avail. Save
134533 100 µg £114
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134534 1 mg £454
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Description

CD66a, known as CEACAM1a, carcinoembryonic antigen-related cell adhesion molecule 1a, is a glycoprotein of the immunoglobulin superfamily and the carcinoembryonic antigen family. Isoforms expressing either two or four alternatively spliced Ig-like domains in mice have been found in a number of epithelial, endothelial, or hematopoietic tissues. CEACAM1a functions as an intercellular adhesion molecule, an angiogenic factor, and a tumor cell growth inhibitor. It also serves as a signal regulatory protein influencing B cell receptor complex-mediated activation. The mouse and human CEACAM1a proteins are targets of viral or bacterial pathogens, respectively. It was reported that targeted disruption of the CEACAM1a gene resulting in a partial ablation of the protein in mice led to reduced susceptibility to virus infection. The antibody recognizes the N-terminal domain of murine CEACAM1a, it does not recognize murine CEACAM1b, an allele in SJL mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC-F - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Mab-CC1 clone recognizes the MHV binding domain of CD66a2. Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A592. SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells2.

Application References

(PubMed link indicates BioLegend citation)
  1. Turner BC, et al. 2004. J. Virol. 78 (10):5486
  2. Williams RK, et al. 1990. J. Virol. 64:3817
  3. Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716

Antigen Details

Structure
A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
Distribution

CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.

Function
CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Nakagaki K, et al. 2005. J. Virol. 79(10):6102
2. Greicius G et al. 2003. J. Leukoc. Biol. 74(1):126
3. Hemmila E et al. 2004. J. Virol. 78(18):10156

Gene ID
26365 View all products for this Gene ID
UniProt
View information about CD66a on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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