Ultra-LEAF™ Purified anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
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Product Citations
publications
93_LEAF_051906
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD16/32 (clone 93), followed by anti-rat IgG FITC.
  • 93_LEAF_051906
    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD16/32 (clone 93), followed by anti-rat IgG FITC.
Cat # Size Price Quantity Avail. Save
101329 100 µg £67
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101330 1 mg £91
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101339 5 mg £329
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101340 25 mg £860
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101341 50 mg £1,438
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101342 100 mg £2,066
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Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
For 5 mg sizes and larger, the antibody is bottled at the concentration indicated on the vial, typically between 1 mg/ml and 3 mg/ml. For smaller sizes the concentration is 1.0 mg/ml.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Block - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (≤1.0 µg per 106 cells in 100 µl volume) for 5-10 minutes on ice prior to immunostaining. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101310). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
RRID
AB_2783037 (BioLegend Cat. No. 101329)
AB_2561482 (BioLegend Cat. No. 101330)
AB_2616683 (BioLegend Cat. No. 101339)
AB_2616684 (BioLegend Cat. No. 101340)
AB_2783038 (BioLegend Cat. No. 101341)
AB_2783039 (BioLegend Cat. No. 101342)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Can I an isotype control with a lambda light chain be substituted with an isotype control with a kappa light chain for flow cytometry?

Yes, you can since kappa and lambda represent light chains which don't contribute to the background staining.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house unless otherwise indicated. However upon request these can be tested from outside independent laboratories on custom basis.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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