Ultra-LEAF™ Purified anti-human CXCL2 (Groβ) Antibody

Pricing & Availability
Clone
A16108E (See other available formats)
Regulatory Status
RUO
Other Names
Gro2, Macrophage inflammatory protein (MIP-2)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16108E_ULEAF_CXCL2_Grobeta_Antibody_071221.png
Recombinant human CXCL2 (Groβ) (Cat. No. 582006) (black circles), induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL2 (Groβ) antibody (clone A16108E) (purple squares) neutralizes the chemotaxis of recombinant human CXCL2 (Groβ) at 0.8 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.2 - 1 µg/mL.
  • A16108E_ULEAF_CXCL2_Grobeta_Antibody_071221.png
    Recombinant human CXCL2 (Groβ) (Cat. No. 582006) (black circles), induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL2 (Groβ) antibody (clone A16108E) (purple squares) neutralizes the chemotaxis of recombinant human CXCL2 (Groβ) at 0.8 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG2b, κ isotype ctrl antibody (Cat. No. 400366) (gray triangles) does not have the effect. ND50 range: 0.2 - 1 µg/mL.
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311001 100 µg £168.00
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311002 1 mg £462.00
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Description

CXCL2 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor; this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8). CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and it has been detected in serum of patients with this disease. Also, CXCL1, CXCL2 and CXCL3 have been shown to be highly expressed in patients with malignant melanoma. In addition, CXCL2 and CXCL1 are upregulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, it has been shown that transfer of encephalitogenic CD4+ Th17 cells induces CXCL1 and CXCL2 transcription in the spinal cords of naïve, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in MS and EAE. In fact, it has been shown that CXCL1 and CXCL2 are upregulated in the preclinical and clinical stages of EAE.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CXCL2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL2 (Groβ) (1 ng/mL) on mouse Baf3-mCXCR2 transfectant chemoattractant cells. ND50 range: 0.2 - 1 µg /mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894458 (BioLegend Cat. No. 311001)
AB_2894458 (BioLegend Cat. No. 311002)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, monocytes, fibroblasts, and epithelial cells.

Function
CXCL2 chemoattracts and activates neutrophils, and it plays a key role in neutrophil mobilization from the bone marrow. CXCL2 is induced by TNFα, IL-1α, IL-17, and LPS.
Interaction
Neutrophils, basophils, lymphocytes, monocytes, keratinocytes, hematopoitic cells, endothelial cells, bone marrow endolthelial cells, osteoblasts, epithelial cells, fibroblasts, melanoma cells, melanocytes, and neurons
Ligand/Receptor
CXCR2
Cell Type
Epithelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Haskill S, et al. 1990. P. Natl. Acad. Sci. USA 87:7732.
2. De Plaen I, et al. 2006. Immunology 118: 153.
3. Carlson T, et al. 2008. J. Exp. Med. 205:811.
4. Eash KJ, et al. 2010. J. Clin. Invest. 120:2423.
5. Keeley EC, et al. 2011. Exp. Cell. Res. 317: 685.
6. Onishi RM and Gaffen SL, 2011. Immunology 129:311.

Gene ID
2920 View all products for this Gene ID
UniProt
View information about CXCL2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/13/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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