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Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
/Poly4053_SPKYG_570_IgG_Antibody_2_011322
Human peripheral blood lymphocytes stained with purified anti-human CD8a (filled histogram) or mouse IgG1, κ purified isotype control (open histogram), followed by anti-mouse IgG Spark YG™ 570.
  • /Poly4053_SPKYG_570_IgG_Antibody_2_011322
    Human peripheral blood lymphocytes stained with purified anti-human CD8a (filled histogram) or mouse IgG1, κ purified isotype control (open histogram), followed by anti-mouse IgG Spark YG™ 570.
  • Poly4053_SPKYG_570_IgG_Antibody_1_011322
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD8a at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue).
Compare all formats See Spark YG™ 570 spectral data
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405338 100 µg £126
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
RRID
AB_2910462 (BioLegend Cat. No. 405338)

Related FAQs

There are no FAQs for this product.

Other Formats

View All IgG Reagents Request Custom Conjugation
Description Clone Applications
APC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Biotin Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
FITC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
HRP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,IHC,WB
PE Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Purified Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,FC,WB,IHC
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC,IHC-F,IHC-P
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-F
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 IHC-P,IHC-F,ICC
Go To Top Version: 1    Revision Date: 01/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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