Recombinant Rat IL-5 (carrier-free)

Pricing & Availability
Other Names
Interleukin 5, Eosinophil Differentiation Factor (EDF), T-Cell Replacing Factor (TRF), Colony-Stimulating Factor (Eosinophil), B-Cell Differentiation Factor II (BCDF-II), Cytotoxic T-lymphocyte inducer.
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Product Citations
publications
Rat_IL-5_RECOM_CF_1_110718_updated
Rat IL-5 induces the proliferation of TF-1 human erythroleukemic cells in a dose dependent manner. EC50 for this effect is 0.5 – 2.5 ng/mL.
  • Rat_IL-5_RECOM_CF_1_110718_updated
    Rat IL-5 induces the proliferation of TF-1 human erythroleukemic cells in a dose dependent manner. EC50 for this effect is 0.5 – 2.5 ng/mL.
  • Rat_IL-5_RECOM_CF_2_110718_updated
    Stability testing for rat IL-5. Recombinant rat IL-5 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (control), and another was frozen and thawed four times (4x freeze/thaws). After this procedure, the samples were tested for their ability to induce TF-1 cell proliferation.
Cat # Size Price Quantity Avail. Save
778502 10 µg £124
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778504 25 µg £208
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778506 100 µg £638
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778508 500 µg £1,680
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Description

IL-5 is a homodimeric glycoprotein that was initially identified by its ability to support the in vitro growth and differentiation of mouse B cells and eosinophils. IL-5 induces eosinophil progenitor cell proliferation, terminal differentiation, and activation. In animal models of allergic diseases or helminth infection, IL-5 induces a massive proliferation of eosinophil progenitors in the bone marrow, promotes eosinophil recruitment with eotaxins, and prolongs eosinophil survival in local tissues. IL-5 regulates genes involved in B cell terminal differentiation. Thus, IL-5 induces CD38-activated splenic B cells to differentiate into immunoglobulin M-secreting cells and go through m to g1 class switch recombination at the DNA level, resulting in immunoglobulin G1 (IgG1) production. IL-5 binds the IL-5R complex, which consists of an IL-5Rα chain specific for IL-5 and a common β-chain that is shared by the receptors for IL-3 and GM-CSF. The alpha subunit is required for ligand-specific binding whereas association with the beta subunit results in increased binding affinity. IL-5 plays important roles in the pathogenesis of asthma, hypereosinophilic syndromes, and eosinophil-dependent inflammatory disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Source
Rat IL-5, amino acids (Met20 - Val132) (Accession # Q08125 ) was expressed in 293E cells. The amino-terminal contains 8His-GGGSIEGR tag
Molecular Mass
The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.1kD. The DTT-reduced and non-reduced protein migrate at approximately 20 -28kD and 45 - 50kD respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20µC to -70µC. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Rat IL-5 induces the proliferation of TF-1 human erythroleukemic cells in a dose dependent manner. The proliferation was measured using Deep Blue Cell Viability™ Kit (Cat. No. 424701). The ED50 = 0.5 - 2.5 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Activated Th2 cells, mast cells, eosinophils, and basophils. In addition, newly identified IL-5 producing cells are: natural helper cells or nuocytes (lately identified as type II innate lymphoid cells), MPPtype2, and Ih2 cells.

Function
IL-5 regulates the production of eosinophils from purified hematopoieitic progenitors and regulates genes involved in B cell terminal differentiation. IL-25 and IL-33 induce Th2 cytokines, among them IL-5.
Interaction
Eosinophils, B cells, basophils, and activated T cells.
Ligand Receptor
Heterodimer IL-5Rα (CD125); β-subunit (CDw131). The β-subunit is shared with IL-3R and GM-CSFR.
Bioactivity
Induces the proliferation of TF-1 cells.
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Lopez AF, et al. 1988. J Exp Med. 167:219.
  2. Horikawa K and Takatsu K, 2006. Immunology. 118:497.
  3. Moro K, et al. 2010. Nature. 463:540.
  4. Neill DR, et al. 2010. Nature. 464:1367.
  5. Saenz SA, et al. 2010. Nature. 464:1362.
  6. Ikutai M, et al. 2012. J Immunol. 188:703.
  7. Yazuda K, et al. 2012. P Nat Acad Sci USA. 109:3451.
Gene ID
24497 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 11/07/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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