- Other Names
- Apolipoprotein H, APOH, Apo-H, Beta-2-glycoprotein 1, B2GPI, Beta(2)GPI, BG, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor
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- Product Citations
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Apolipoprotein H (ApoH) is a single-chain plasma protein of about 50 kD. It is a member of the complement control superfamily of proteins. ApoH is also known as beta-2-glycoprotein 1 and activated protein C-binding protein. ApoH has been implicated in a variety of physiologic pathways, including blood coagulation, hemostasis, and the production of antiphospholipid antibodies characteristic of antiphospholipid syndrome. ApoH binds negatively charged phospholipid macromolecules and circulates as a component of lipoprotein particles and as a lipid-free serum protein. ApoH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. ApoH also inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows pro-coagulant activity by inhibiting the activation of Protein C. The plasma concentrations of ApoH are strongly associated with metabolic syndrome alterations and vascular disease in type 2 diabetics and could be considered as a clinical marker of cardiovascular risk.Product Details
- Mouse ApoH, amino acid Gly20-Cys345 (Accession # BC019811), with a C-terminal 8x His tag, was expressed in 293E cells.
- Molecular Mass
- The 339 amino acid recombinant protein has a predicted molecular mass of approximately 38.1 kD. The DTT-reduced protein migrates at approximately 60 kD and and non-reduced protein migrates at approximately 55 kD by SDS-PAGE. The predicted N-terminal amino acid is Gly.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- When mouse Apolipoprotein H/ApoH is coated at 2 µg/mL (100 µL/well), biotinylated mouse LDLR binds with an EC50 of 0.1 – 0.4 µg/mL in a functional ELISA.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
Expressed by the liver and secreted in plasma
- Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate
- Heparin, phospholipids, dextran sulfate
- Measured by its ability to bind mouse LDLR
- Biology Area
- Cell Biology
- Molecular Family
- Antigen References
- Hoeg JM, et al. 1985. Atherosclerosis. 55:25-34
- Bancsi LF, et al. 1992. Thromb Haemost. 67:649-653
- Chonn A, et al. 1995. J Biol Chem. 270:25845-25849
- Sheng Y, et al. 2001. J Biol Chem. 276:13817-13821
- Shi T, et al. 2004. Proc Natl Acad Sci. 101:3939-3944
- Castro A, et al. 2010. Atherosclerosis. 209:201-205
- Giannakopoulos B, et al. 2011. Curr Rheum Rep. 13:90-95
- Gene ID
- 11818 View all products for this Gene ID
- View information about Apolipoprotein H on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.