Recombinant Human MICA-Fc Chimera (carrier-free)

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Other Names
MHC class I polypeptide-related sequence A, Truncated MHC Class I Polypeptide-Related Sequence A, MHC Class I Chain-Related Protein A, Stress Inducible Class I Homolog, HLA Class I Antigen, PERB11.1, MIC-A
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Human_MICA_RECOM_CF_1_052119
Recombinant human MICA binds to immobilized recombinant human NKG2D in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 1 - 5 µg/mL.
  • Human_MICA_RECOM_CF_1_052119
    Recombinant human MICA binds to immobilized recombinant human NKG2D in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 1 - 5 µg/mL.
  • Human_MICA_RECOM_CF_2_052419_updated
    Stability Testing for Recombinant Human MICA. Recombinant human MICA was aliquoted in PBS, pH 7 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaws) and compared to the control that was kept at 4°C (control). The samples were tested for their ability to bind to immobilized NKG2D in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 1 - 5 µg/mL.
Cat # Size Price Quantity Avail. Save
782702 10 µg £64
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782704 25 µg £101
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782706 100 µg £319
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782708 500 µg £806
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Description

MICA, human major histocompatibility complex (MHC) class I polypeptide-related sequence A is a member of the MIC family that includes MICA, MICB, and five pseudogenes MICC to MICG. MICA is a protein of 383 amino acids and interacts with the NKG2D receptor and the T cell receptor (TCR) to activate NK cells, αβ CD8 T cells, and γδ T cells. MICA and MICB contain two Ig-like domains (α1, α2) and the C-type Ig-like α3 domain, a transmembrane domain, and a carboxyl-terminal intracellular tail. MICA and MICB are conserved in most mammals, but not in rodents. MICA and MICB have high similarity (84% identical). MICA and MICB diverge from the MHC class I family, when they are compared domain by domain, they present a similarity of 28 to 35%. MICA and MICB bind to NKG2D, and the binding of MICB to the receptor can be blocked by a chimeric form of UL16; indicating that UL16 binds to the same, or overlaps with the binding epitope present in NKG2D on the NK cells. MICA and MICB, and the UL16-binding proteins are expressed in the membrane of tumor cells, and the participation of ADAM10 and ADAM17 has been associated to shedding of these NKG2D ligands. Soluble NKG2D ligands downregulate NKG2D expression in the effector lymphocytes; therefore, helping the tumor to escape.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Host Species
CHO cells
Source
Human MICA, amino acid Glu24 - Trp308 (Accession # Q29983) was expressed in CHO cells. The carboxyl terminus contains SRIG-Fc 6His tag.
Molecular Mass
The 529 amino acid recombinant protein has a predicted molecular mass of approximately 62.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 80 kD and 160 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Glu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human MICA binds to recombinant human NKG2D in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 1 - 5 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Gastrointestinal epithelium, stressed airway epithelial cells, fibroblasts, cancer cell lines, cervical cancer, myelomonocytic leukemia, multiple myelomas and lymphomas.

Function
Interaction of MICA with NKG2D triggers cytotoxicity and cytokine production. MICA plays a key role in innate and adaptive immune responses. Induced upon cellular stress, viral infection, or malignant transformation.
Interaction
NK cells, CD8 αβT cells, and γδ T cells
Ligand/Receptor
NKG2D, NKG2D/DAP10 complex
Bioactivity
Recombinant human MICA binds to recombinant NKG2D in a dose-dependent manner using a functional ELISA.
Cell Type
Epithelial cells, NK cells, T cells
Biology Area
Adaptive Immunity, Cell Adhesion, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, MHC Antigens
Antigen References
  1. Steinle A, et al. 2001. Immunogenetics 53:279.
  2. Holmes MA, et al. 2002. J. Immunol. 169:1395.
  3. Zocchi MR, et al. 2012. Blood 119:1479.
  4. Wu BJ, et al. 2013. Tumour. Biol. 34:565.
  5. Zocchi MR, et al. 2016. OncoImmunology 5: e1123367.
  6. Zuo J, et al. 2018. Front Immunol. fimmu.2018.01820.
  7. Lazarova M, Steinle A. 2019. Expert Opin Ther Targets. 23:281-294.
Gene ID
100507436 View all products for this Gene ID
UniProt
View information about MICA on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 05/21/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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