Recombinant Human IL-33 (Animal-Free)

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-1F11, Nuclear factor from high endothelial venules (NF-HEV)
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Product Citations
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716302 10 µg £174
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Description

IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1β. IL-33, IL-1β, and IL-18 are synthesized as biologically inactive precursors and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and, subsequently, activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1β. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human IL-33, amino acids Ser112-Thr270 (Accession# NP_ 118656), was expressed in E. coli.
Molecular Mass
The 159 amino acid recombinant protein has a predicted molecular mass of 16.9 kD. The predicted N-terminal amino acid is Ser.
Purity
>98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2.0 x 107 units/mg as determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.
Application

Bioassay

Application Notes

This product is reactive with human and mouse.

Antigen Details

Structure
Cytokine
Distribution
IL-33 mRNA is expressed in a broad range of human tissues, such as heart, small intestine, brain, ovary, testis, pancreas, and colon. Higher IL-33 expression was detected in human keratinocyte, fibroblast, myocyte, and alveolar cells.
Function
IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-α and IL-1β are activators of IL-33 transcription in fibroblasts and keratinocytes.
Interaction
Th2 cells
Ligand/Receptor
IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
Bioactivity
Human IL-33 is able to induce proliferation of mouse D10S cells.
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schmitz J, et al. 2005. Immunity 23:479.
2. Barksby HE, et al. 2007. Clin. Exp. Immunol. 149:217.
3. Arend WP, et al. 2008. Immunol. Rev. 223:20.
4. Suzukawa M, et al. 2008. J. Immunol. 181:5981.
5. Moussion C, et al. 2008. PLoS One 3:e3331.
6. Mildner M, et al. 2010. Cardiovasc. Res. 87:769.

Gene ID
90865 View all products for this Gene ID
UniProt
View information about IL-33 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 3    Revision Date: 05/06/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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