- Other Names
- Carcinoembryonic antigen-related cell adhesion molecule 8, CEACAM-8, CEACAM8, CD67 antigen, CD66b, Carcinoembryonic antigen CGM6, Nonspecific cross-reacting antigen NCA-95
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- Product Citations
CEACAM-8, also known as CD66b and CD67, is a GPI-linked protein belonging to the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. A homolog of CEACAM-8 has not been identified in rodents. CEACAM-8 is exclusively expressed on human granulocytes, including neutrophils and eosinophils, and is characterized as a granulocyte activation marker. CEACAM-8 is mainly found in secondary granules with low amounts on cell the surface. Upon activation of granulocytes with various stimuli, CEACAM-8 is rapidly upregulated and released from intracellular granules, leading to a high level of expression on the plasma membrane. CEACAM-8 has been reported to be involved in immune response, leukocyte migration, cell adhesion and degranulation. Engagement of CEACAM-8 with Galectin-3 activates a Src kinase, Hck, inducing cell adhesion and degranulation. In addition, CEACAM-8 constitutively associates integrin β2 and CD11b in lipid rafts, regulating cell adhesion and activation of eosinophils. CEACAM-8 can specifically bind CEACAM-6 (CD66c) through their Ig-like V-type domain in calcium-independent manner. The interaction of CEACAM-8 and CEACAM-6 mediates heterophilic cell adhesion.Product Details
- Antibody Type
- Human CEACAM-8, amino acid (Gln35-Asp320) (Accession: #P31997.2) with a C-terminal 6x His tag, was expressed in 293E cells.
- Molecular Mass
- The 292 amino acid recombinant protein has a predicted molecular mass of approximately 32.3 kD. The DTT-reduced and non-reduced protein migrates at approximately 60 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to -70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- When human Galectin-3 (Cat. No. 599704) is immobilized at 2 µg/mL, human CEACAM-8/CD66b binds with EC50 of 50 - 200 ng/mL in a functional ELISA.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org.
- IgG superfamily
Restricted to bone marrow, granulocytes; secondary granules, plasma membrane, GPI-anchored
- Cell adhesion, activation of neutrophils and eosinophils
- CEACAM-6 (CD66c), CD11b
- Ligand Receptor
- Measured by Galectin-3 binding assay
- Biology Area
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
- Kuijpers T, et al. 1993. J Immunol. 151: 4934.
- Kuroki M, et al. 1992. J Leuk Biol. 52: 551.
- Ducker TP, et al. 1992. J Leuk Biol. 52: 11.
- Yoon J, et al. 2007. J Immunol. 179: 8454.
- Oikawa S, et al. 2000. Biochem Biophys Res Commun. 278: 564.
- Zhao L, et al. 2002. J Immunol Methods. 270: 27.
- Yamanka T, et al. 1996. Biochem Biophys Res Commun. 219: 842.
- Oikawa S, et al. 1991. J Biol Chem. 266: 7995.
- Gene ID
- 1088 View all products for this Gene ID
- View information about CEACAM-8/CD66b on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.