Recombinant Human CD58 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 3, LFA-3, Ag3
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Product Citations
publications
Recom_Human_CD58_CF_1_101223
Immobilized Recombinant Human CD58 induces adhesion of Jurkat cells in a dose-dependent mannner. The ED50 for this effect is 2.5 – 15 µg/mL.
  • Recom_Human_CD58_CF_1_101223
    Immobilized Recombinant Human CD58 induces adhesion of Jurkat cells in a dose-dependent mannner. The ED50 for this effect is 2.5 – 15 µg/mL.
  • Recom_Human_CD58_CF_2_101223
    Stability testing for Recombinant Human CD58. Recombinant Human CD58 was aliquoted in PBS, pH 7.0 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to induce adhesion of Jurkat cells in a dose-dependent manner. The ED50 for this effect is 2.5 – 15 µg/mL.
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565904 25 µg £236
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565906 100 µg £469
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Description

CD58, also known as LFA-3, is a type I transmembrane protein belonging to the CD2 family. Alternative splicing of LFA-3 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. LFA-3 is expressed on both hematopoietic and non-hematopoietic cells including: B cells, T cells, monocytes, dendritic cells, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. LFA-3 expressed on antigen presenting cells enhances MHC class I and II-restricted T cell antigen recognition via the binding of CD2 on the T cell surface. The interaction between CD2 and LFA-3 also mediates thymocyte interaction with thymic epilthelial cells and T cell rosetting with erythocytes. CD58 serves as a co-stimulatory molecule to induce IL-10 and IFN-γ production by human T cells. Recombinant CD58/LFA-3 can serve as a modulator to block E-rosette and NK cytotoxicity, and synergize T cell activation with antibodies against CD2. The CD58-CD2 axis is coregulated with PD-L1 via CMTM6 (CKLF-like MARVEL transmembrane domain-containing 6) and shapes anti-tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD58, amino acids Phe29-Arg215 (Accession # P19256.1), with a C-terminal 10x His tag, was expressed in 293E cells.
Molecular Mass
The 197 amino acid recombinant protein has a predicted molecular mass of approximately 22.8 kD. The DTT-reduced and non-reduced protein migrates at approximately 45-55 kD by SDS-PAGE. The predicted C-terminal amino acid is His.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS
Endotoxin Level
Less than 0.1 EU per µg of cytokine as determined by the LAL method.
Concentration
25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at - 20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized Recombinant Human CD58 supports the adhesion of Jurkat cells in presence of 160 nM PMA. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. EC50 of this binding is 2.5 – 15 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Ig-like protein
Distribution

Expressed on both hematopoietic and non-hematopoietic cells including: B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells.

Function
Mediates adhesion between various cell types (target cells and cytotoxic cells, APC and T cells, thymocytes and thymic epithelia)
Interaction
T cells and NK cells
Ligand/Receptor
CD2, CMTM6
Bioactivity
Immobilized Recombinant Human CD58 supports the adhesion of Jurkat cells in presence of 160 nM PMA. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. EC50 of this binding is 2.5-15 µg/mL.
Cell Type
B cells, Endothelial cells, Epithelial cells, Erythrocytes, Fibroblasts, Monocytes, T cells
Biology Area
Cell Adhesion, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Arulanandam AR, et al. 1993. Proc Natl Acad Sci USA. 90:11613-7.
  2. Davis SJ, et al. 1996. Immunol Today. 17:177-87.
  3. Smith MEF, et al. 1990. J Clin Patho. 43:893-900.
  4. Selvaraj P, et al. 1987. Nature. 326:400-3.
  5. Vollger LW, et al. 1987. J Immunol. 138:358-63.
  6. Plunkett ML, et al. 1987. J Exp Med. 165:664-76. 
  7. Albert-Wolf M, et al. 1991. Int Immunol. 3:1335-47
  8. Dustin ML, et al. 1997. J Biol Chem. 272:30889-98.
  9. Wang JH, et al. 1999. Neurochem Res. 25:613-9.
  10. Bullens DMA, et al. 2001. Int Immunol. 13:181-91.
  11. Rölle A, et al. 2016. Eur J Immunol. 46:2420-2425
  12. Springer TA, et al. 1987. Annu Rev Immunol. 5:223-52.
  13. Dustin ML, et al. 1987. Nature. 329:846-8.
  14. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861-71.
  15. Sanders ME, et al 1988. J Immunol. 140:1401-7.
  16. Zhu DM et al. 2006. ACS Chem Biol. 1:649-58. 
  17. Patricia Ho, et al. 2023. Cancer Cell. 41:1207-1221.
Gene ID
965 View all products for this Gene ID
UniProt
View information about CD58 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 10/12/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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