Recombinant Human CD23/FCER2 (carrier-free)

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Other Names
FCER2, Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, Immunoglobulin E-binding factor, IGEBF, C-type lectin domain family 4 member J, CLEC4J, low affinity IgE receptor, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor
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Human_CD23_FCER2_CF_RECOM_1_042219
When human CD23 is immobilized at 2.0 µg/mL, human IgE binds with an EC50 of 0.1 - 0.4 µg/mL. Biotin anti-human IgE antibody (Cat. No. 325503) was used for detection.
  • Human_CD23_FCER2_CF_RECOM_1_042219
    When human CD23 is immobilized at 2.0 µg/mL, human IgE binds with an EC50 of 0.1 - 0.4 µg/mL. Biotin anti-human IgE antibody (Cat. No. 325503) was used for detection.
  • Human_CD23_FCER2_CF_RECOM_2_042219
    Stability testing for Human CD23. Human CD23 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested in a binding assay with human IgE.
Cat # Size Price Quantity Avail. Save
782502 10 µg £60
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782504 25 µg £84
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782506 100 µg £289
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782508 500 µg £739
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Description

CD23 is a 45 kD type II transmembrane glycoprotein that is a member of the subgroup of the C-type lectin superfamily. CD23 is a low affinity receptor for IgE, CR2/CD21, CD11b, and CD11c. CD23 is expressed by a wide variety of cell types and has essential function in the regulation of IgE production and in the differentiation of B cells. Soluble CD23 induces IgE secretion by binding to CD21. On the other hand, secreted IgE will bind CD23 on B-cell membranes, preventing its cleavage, and thus shutting down further IgE production. In human monocytes, CD23 interaction with CD11b and CD11c results in the release of pro-inflammatory cytokines. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. Both soluble and membrane bound CD23 show bioactivity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Host Species
CHO cells
Source
Human CD23, amino acid Asp48-Ser321 (Accession # P06734) with a C-terminal 6x His tag, was expressed in CHO cells.
Molecular Mass
The 284 amino acid recombinant protein has a predicted molecular mass of approximately 32.1 kD. Both the DTT-reduced and non-reduced proteins migrate at approximately 40 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per μg protein as determined by the LAL method.
Concentration
10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When human CD23 is immobilized at 2.0 µg/mL, human IgE binds with an EC50 of 0.1 - 0.4 µg/mL. Biotin anti-human IgE Antibody (Cat. No. 325503) was used for detection.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution

B cells, widely expressed with highest expression in adrenal gland

Function
Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Interaction
B cells, monocytes
Ligand/Receptor
IgE, CD21, CD11b, CD11c
Bioactivity
Measured in a binding assay with human IgE
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors, Soluble Receptors
Antigen References
  1. Delespesse G, et al. 1989. J. Immunol. 142:134-8.
  2. Lecoanet-Henchoz S, et al. 1995. Immunity. 3:119-25.
  3. Hibbert RG, et al. 2005. J. Exp. Med. 202:751-60.
  4. Weskamp G, et al. 2006. Nat. Immunol. 7:1293-8.
  5. Wurzburg BA, et al. 2006. Structure. 14:1049-58.
Gene ID
2208 View all products for this Gene ID
UniProt
View information about CD23/FCER2 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 04/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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