Purified Mouse IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-173 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MOPC-173_GoChIP_IgG2a_Antibody_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 HeLa cells with either A)1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II RPB1 antibody (clone 8WG16, Cat. No. 664911) or B) equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype control antibody (clone MOPC-173, Cat. No. 400287) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MOPC-173_GoChIP_IgG2a_Antibody_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 HeLa cells with either A)1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II RPB1 antibody (clone 8WG16, Cat. No. 664911) or B) equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype control antibody (clone MOPC-173, Cat. No. 400287) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400201 50 µg £17.00
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400202 500 µg £75.00
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Description

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).

Product Citations
  1. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  2. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  3. Woo CM, et al. 2018. Mol Cell Proteomics. 17:764. PubMed
  4. Nelde A, et al. 2018. Oncoimmunology. 7:e1316438. PubMed
  5. Dias J, et al. 2017. Proc Natl Acad Sci U S A. 114:E5434. PubMed
  6. Nakajima NI, et al. 2017. Oncol Rep. 38:693. PubMed
  7. Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed
  8. Lever JM, et al. 2019. JCI Insight. 4:e125503. PubMed
  9. Wosen JE, et al. 2019. Front Immunol. 1.784722222. PubMed
  10. Barthélémy F, et al. 2019. Mol Ther Nucleic Acids. 1.152777778. PubMed
  11. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  12. Heger L, et al. 2018. Front Immunol. 9:744. PubMed
  13. álvaro-Benito M, et al. 2020. Cell Mol Immunol. 17:133. PubMed
  14. Serwas NK, et al. 2019. Nat Commun. 2.573611111. PubMed
  15. Podolin P, et al. 2008. J Immunol. 180:7989. PubMed
  16. Ohno Y, et al. 2013. J Biochem. 154:355. PubMed
  17. Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
  18. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
  19. Elias S, et al. 2020. Haematologica. . PubMed
  20. Drummond E, et al. 2020. Brain. 2803:143. PubMed
  21. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?
IgG2a gene is deleted in C57Bl/6, C57Bl/ 10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Go To Top Version: 2    Revision Date: 08/24/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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