Purified anti-VPS35 Antibody

Pricing & Availability
Clone
A17084A (See other available formats)
Other Names
Vacuolar protein sorting-associated protein 35
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A17084A_Pure_VPS35_Antibody_1_102418
Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HEK293 lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17084A_Pure_VPS35_Antibody_1_102418
    Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of HEK293 lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17084A_Pure_VPS35_Antibody_2_102418
    Western blot of purified anti-VPS35 antibody (clone A17084A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 ng of recombinant human VPS35 protein. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
856401 25 µg £64
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856402 100 µg £165
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Description

VPS35 is the largest subunit of the retromer complex, which plays an important role in retrograde protein transport from the endosome to the trans-Golgi network. VPS35 binds to VPS26 (VPS26A or VPS26B) and VPS29 to form the vacuolar protein sorting subcomplex (VPS), which in turn interacts with different nexins to form different retromer variants that transport different targets. D620N mutation in VPS35 has been implicated as a causative genetic factor for developing late-onset, autosomal dominant familial Parkinson’s disease, and could serve as potential therapeutic target for PD.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783456 (BioLegend Cat. No. 856401)
AB_2783457 (BioLegend Cat. No. 856402)

Antigen Details

Structure
VPS35 is a 796 amino acid protein with an apparent molecular mass of ~92 kD.
Distribution

Tissue Distribution: Ubiquitously expressed in all tissues.
Cellular Distribution: Cytosol, lysosome, endosome, and secreted.

Function
Largest subunit of the retromer complex involved in retrograde transport of proteins from endosomes to the trans-Golgi network.
Interaction
VPS26 (VPS26A or VPS26B), VPS29, SNX1, SNX2, IGF2R, SNX3, GOLPH3, LRRK2, SLC11A2, SNX27, RAB7A.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Organelle Markers
Antigen References
  1. Cui Y, et al. 2018. FEBS Lett. 592(7):1096
  2. Williams ET, et al. 2017. J Parkinsons dis. 7(2):219
  3. Ferreira M, et al. 2017. Acta Neurol Scand. 135(3):273
Gene ID
55737 View all products for this Gene ID 65114 View all products for this Gene ID 25479 View all products for this Gene ID
UniProt
View information about VPS35 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/24/2018

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