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Purified anti-STING (TMEM173) Antibody

Pricing & Availability
Clone
O94E12 (See other available formats)
Regulatory Status
RUO
Other Names
Transmembrane Protein 173, MPYS, Endoplasmic Reticulum Interferon Stimulator, ERIS, MITA, NET23, and Mitochondrial Mediator of IRF3 Activation
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_O94E12_PURE_STING_TMEM173_Antibody_WB_112817
Western blot analysis of cell lysates from THP-1 (positive control) and HEL (low expression negative control) cells using STING Mouse primary antibody (clone O94E12, 1:500 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_O94E12_PURE_STING_TMEM173_Antibody_WB_112817
    Western blot analysis of cell lysates from THP-1 (positive control) and HEL (low expression negative control) cells using STING Mouse primary antibody (clone O94E12, 1:500 dilution) and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
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675902 100 µg £182
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Description

STING (TMEM173), also called stimulator of interferon genes, is a transmembrane adaptor protein that plays an important role in the innate immune response against viral and bacterial infections. It has been shown that cyclic GMP-AMP synthase (cGAS) plays a key role in STING activation. Upon activation, STING triggers downstream transcription factors STAT6 and IRF3 through TBK1 and leads to production of IFN-β. IFN-β protects infected cells and nearby cells from local infection. It is clear that chronic STING activation is involved in chronic inflammatory diseases, such as autoimmune syndromes and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant STING (TMEM173) protein produced in 293T cells.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml (1:250-1:500). It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Serrano R, et al. 2022. Oncoimmunology. 11:2030021. PubMed
  2. Kabelitz D, et al. 2022. Sci Rep. 12:17827. PubMed
RRID
AB_2565608 (BioLegend Cat. No. 675902)

Antigen Details

Structure
A 379 amino acid protein with a predicated molecular weight around 42 kD, and observed molecular weight on SDS gel is around 35 kD.
Distribution

STING is expressed in various hematopoietic cells such as T lymphocytes, NK cells, myeloid cells and monocytes and is also highly expressed in lung, ovary, heart, smooth muscle, retina, bone marrow and vagina.

Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.
Interaction
MHC-II complex, DDX58/RIG-I, MAVS, SSR2, RNF5, TRIM56, TBK1, IFIT1 and IFIT2.
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Lemos H, et al. 2015. Expert Rev Clin Immunol. 11:155.
2. Konno H, et al. 2014. Microbes Infect. 16:998.
3. Maringer K, et al. 2014. Cytokine Growth Factor Rev. 25:669.
4. Cai X, et al. 2014. Mol Cell. 54:289.
5. Barber GN, et al. 2014. Trends Immunol. 35:88.
6. Sun L, et al. 2013. Science. 339:786.

Gene ID
340061 View all products for this Gene ID
UniProt
View information about STING on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/10/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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