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Purified anti-SP1 Antibody

Pricing & Availability
Clone
W21043E (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor sp1, TSFP1, Specificity Protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21043E_PURE_SP1_WB_082323
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-SP1 (clone W21043E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W21043E_PURE_SP1_WB_082323
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-SP1 (clone W21043E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W21043E_PURE_SP1_ICC_082323
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1.25 µg/mL of either purified anti-SP1 (clone W21043E) (panel B) or purified rat IgG2a, κ isotype control (Cat. No. 400502) (panel A) followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X oil objective. Scale bar: 50 µm
  • C. W21043E_PURE_SP1_IHCP_082323
    IHC staining using purified anti-SP1 (clone W21043E) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Tris-EDTA buffer (10 mM Tris, 1 mM EDTA, pH 9.0), the tissue was incubated without (panel A) or with (panel B) 1.0 µg/mL of purified anti-SP1 (clone W21043E) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with 40X objective. Scale bar: 50 µm
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615251 25 µg £133
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615252 100 µg £331
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Description

SP1 belongs to the transcription factor SP/KLF family, which contains three zinc finger domains that can bind to GC boxes and regulate transcription of a variety of molecules. SP1 regulates efferent gene transcription positively and negatively, participating broadly in cellular development and physiological functions as a generally expressed transcription factor. Post-translational modifications, including phosphorylation, glycosylation, acetylation, ubiquitylation, and SUMOylation, highly regulate SP1’s function and stability. SP1 is required to develop and differentiate early embryo, placenta, and sperm cells. SP1 is highly expressed in multiple cancers including breast, stomach, pancreas, lung, and brain. SP1 promotes tumor cell proliferation, inactivates cell death, and facilitates immune escape.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli derived recombinant fragment of human SP1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The mouse cross-reactivity of this product has been verified by WB.

For use in immunohistochemistry (IHC), antigen retrieval with Tris-EDTA pH 9.0 is recommended.

For use in immunocytochemistry (ICC), paraformaldehyde fixation is recommended.

RRID
AB_3083401 (BioLegend Cat. No. 615251)
AB_3083401 (BioLegend Cat. No. 615252)

Antigen Details

Structure
SP1 is a 785 amino acid protein with an expected molecular weight of about 80.6 kD.
Distribution

Nuclear/Ubiquitous

Function
Binds to GC boxes to regulate transcriptional activity
Interaction
EP300, E2F1, RELA, HDACs, SMADs, BRCA1, BRCA2, RNF4, c-MYC, c-JUN, p53
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Immunology, Neurodegeneration, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Saffer JD, et al. 1991. Mol Cell Biol. 11:2189-99.
  2. Thomas K, et al. 2007. Mol Cell Endocrinol. 270:1-7.
  3. Vizaino C, et al. 2015. Pharmacol Ther. 152:111-24.
  4. Beishline K, et al. 2015. FEBS J. 282:224-58.
Gene ID
6667 View all products for this Gene ID
UniProt
View information about SP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/25/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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