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Purified anti-SOX2 Antibody

Pricing & Availability
Clone
14A6A34 (See other available formats)
Regulatory Status
RUO
Other Names
SRY-related HMG-box gene 2, SRY (sex determining region Y)-box 2, MCOPS3, ANOP3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_14A6A34_Purified_SOX2_Antibody_WB_1_120417
Total lysates (15 µg protein) from HeLa (Low expression negative control) and NTERA-2 (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (0.25 µg/ml), and 1:1000 (0.5 µg/ml) purified anti-SOX2 antibody, clone 14A6A34. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1_14A6A34_Purified_SOX2_Antibody_WB_1_120417
    Total lysates (15 µg protein) from HeLa (Low expression negative control) and NTERA-2 (Positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 (0.25 µg/ml), and 1:1000 (0.5 µg/ml) purified anti-SOX2 antibody, clone 14A6A34. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2_14A6A34_SOX2_antibody_WB_080316
    Total cell lysate from NTERA-2 cells (lane 1, 15 µg), NF-1 cells (lane 2, 15 µg) and HeLa cells (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-SOX2 antibody (clone 14A6A34). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 3_14A6A34_Purified_SOX2_Antibody_ICC_052419
    NTERA-2 cells were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/mL SOX2 Antibody (14A6A34) and followed by DyLight™ 594 goat anti-mouse IgG (red) for 1 hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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656102 100 µg £213
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Description

SOX2 is the most studied member of SRY-related box transcription factor family. It binds to target genes through its highly conserved HMG box domain. Inactivation of the SOX2 gene causes lethality during embryonic development. SOX2 knockdown in embryonic stem cells results in their differentiation. Co-expression of SOX with OCT4, MYC, and KLF4 is sufficient to reprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similar characteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial for the self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2 has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cycle arrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting that SOX2 is involved in activating genes associated with tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human SOX recombinant protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 0.5 µg per ml (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  2. Patterson K, et al. 2020. Stem Cell Res. 42:101673. PubMed
  3. Kishore S, et al. 2020. Cell Stem Cell. 27(1):137-146.e6. PubMed
  4. Patterson K, et al. 2019. Stem Cell Res. 41:101627. PubMed
RRID
AB_2562246 (BioLegend Cat. No. 656102)

Antigen Details

Structure
317 amino acids, predicted molecular weight of 34 kD, contains a HMG box domain responsible for DNA binding
Distribution

Nucleus

Function
Transcription factor that regulates the expression of the genes involved in embryonic development
Interaction
Interacts with FGFR1, SOX3, and ZSCAN10
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Antigen References

1. Rizzino A. 2009. Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228.
2. Stolzenburg S, et al. 2012. Nucleic Acids Res. 40:6725.
3. Lai YS, et al. 2012. Proc. Natl. Acad. Sci. USA. 109:3772.
4. Jeong CH, et al. 2010. Stem Cells 28:2141.
5. Xiang R, et al. 2011. Br. J. Cancer 104:1410.
6. Card DA, et al. 2008. Mol. Cell Biol. 28:6426.

Gene ID
6657 View all products for this Gene ID
UniProt
View information about SOX2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/04/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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