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Purified anti-SKP1 Antibody

Pricing & Availability
Clone
P84E11C10 (See other available formats)
Regulatory Status
RUO
Other Names
S-Phase Kinase Associated Protein 1, EMC19, OCP-II, OCP2, TCEB1L, p19a, P19skp1, SIII
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P84E11C10_PURE_SKP1_Antibody_051222
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-SKP1 (clone P84E11C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • P84E11C10_PURE_SKP1_Antibody_051222
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-SKP1 (clone P84E11C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
  • P84E11C10_PURE_SKP1_Antibody_2_051222
    HeLa cells were fixed and permeabilized with ice cold MeOH for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 5.0 µg/mL of either purified mouse IgG1, κ isotype control (Cat. No. 400102) (panel A) or purified anti-SKP1 (clone P84E11C10) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective. Scale bar: 50 µm
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605651 25 µg £81
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605652 100 µg £201
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Description

S-Phase Kinase Associated Protein 1 (SKP1) is one of the core components of the Skp, Cullin, F-box containing (SCF) E3 ubiquitin ligase complex which poly-ubiquitinates protein targets for proteasomal degradation, and thereby regulates a variety of cellular processes including DNA damage response, apoptosis, cell cycle, and overall genome stability. Within the SCF, SKP1 serves as an adaptor protein linking the scaffolding protein Cul1 to F-box proteins. Beyond its role as part of SCF, SKP1 also functions in centrosome organization, chromosome segregation, meiotic progression, and oocyte survival. Aberrant SKP1 expression is associated with chromosomal misalignment and instability, disrupted cell cycle, and is a characteristic of several cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human SKP1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC, we recommend fixation with MeOH. Fixation in 4% PFA followed by permeabilization with MeOH, and fixation in 4% PFA followed by permeabilization with 0.5% Triton X-100 were both tested but produced weak staining.

This clone is not recommended for IHC-P. Testing was conducted using formalin-fixed paraffin-embedded human testis but only a faint signal was produced

RRID
AB_2924614 (BioLegend Cat. No. 605651)
AB_2924614 (BioLegend Cat. No. 605652)

Antigen Details

Structure
SKP1 is a 163 amino acid protein with a predicted molecular weight of 19 kD.
Distribution

Localized to the centrosome, cytosol, nucleus, and nucleoplasm/ubiquitously expressed

Function
Host-virus interaction, Ubl conjugation pathway, Ubiquitin/Protein Degradation, Adaptor Protein
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Ubiquitin/Protein Degradation
Molecular Family
GTPases
Antigen References
  1. Piva R, et al. 2002. Mol Cell Biol. 22:8375-87.
  2. Thompson L, et al. 2022. Front Cell Dev Biol. 10:3389.
  3. Yongjuan G, et al. 2020. Sci Adv. 6:22129.
Gene ID
6500 View all products for this Gene ID
UniProt
View information about SKP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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