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Purified anti-Neu1 Antibody

Pricing & Availability
Clone
A18167C (See other available formats)
Regulatory Status
RUO
Other Names
Neuraminidase 1 (NEU1), sialidase 1 (SIAL1), sialidase-1 Acetylneuraminyl hydrolase (NANH), G9 sialidase, Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A18167C_PURE_Neu1_Antibody_1_010824
IHC staining of Purified anti-Neu1 (clone A18167C) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-Neu1 (clone A18167C) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • A18167C_PURE_Neu1_Antibody_1_010824
    IHC staining of Purified anti-Neu1 (clone A18167C) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Purified anti-Neu1 (clone A18167C) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
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623851 25 µg £134
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623852 100 µg £339
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Description

Neu1, a member of the sialidase enzyme family, plays a crucial role in cellular processes by removing sialic acid (N-acetylneuraminic acid) residues from glycoproteins and glycolipids on the cell surface. This process, known as desialylation, impacts various cellular functions, including cell-cell interactions, signal transduction, and immune responses. Additionally, desialylation recycles sialic acid, contributing to the maintenance of cellular sialic acid levels critical for normal physiological processes. Alterations in Neu1 activity leads to sialidosis, a lysosomal storage disease resulting in an accumulation of sugar-protein complexes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C-terminal fragment of recombinant human Neu1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone A18167C) is not suitable for Western blot (WB) or immunocytochemistry (ICC).

For immunohistochemistry (IHC), we recommend antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0). Antigen retrieval with Sodium Citrate is not recommended.

Antigen Details

Structure
Neu1 is a 415 amino acid protein with a predicted molecular weight of 45.5 kD.
Distribution

Cytoplasmic, lysosomal

Function
Sialidase, with a preference for alpha 2-3 and alpha 2-6 sialyl linkage
Molecular Family
Cytokines/Chemokines, Enzymes and Regulators
Antigen References
  1. Khan A & Sergi CM. 2022. Front Pharmacol. 13:902259.
  2. Seyrantepe V, et al. 2003. Hum Mutat. 22:343-52
  3. Toussaint K, et al. 2022. Cancers. 14:4868.
Gene ID
4758 View all products for this Gene ID
UniProt
View information about Neu1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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