Purified anti-mouse / rat IL-1β Antibody

Pricing & Availability
Clone
B122 (See other available formats)
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Armenian Hamster IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
503501 50 µg £44
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503502 500 µg £81
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli -expressed, recombinant mouse IL-1β
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1β sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

Certain formats of clone B122 have been reported for in vitro neutralization of IL-1β bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.

Application References

(PubMed link indicates BioLegend citation)
  1. Hogquist K, et al. 1991. J. Immunol. 146:1534.
  2. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
  3. Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
  4. Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
  5. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
  6. Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
  7. Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
  8. Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed
Product Citations
  1. Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed
Publication Library
RRID
AB_315286 (BioLegend Cat. No. 503501)
AB_315287 (BioLegend Cat. No. 503502)

Antigen Details

Structure
Cytokine; 17.5 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
16176 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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