- Poly5165 (See other available formats)
- Regulatory Status
- Other Names
- Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
- Goat Polyclonal Ig
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- Product Citations
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IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.Product Details
- Mouse, Cross-Reactivity: Rat
- Antibody Type
- Host Species
- Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by antigen affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.
- Application Notes
ELISA Capture: The purified Poly5165 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5165 antibody (Cat. No. 516503) as the detecting antibody and recombinant mouse IL-33 (Cat. No. 580509) as the standard.
(PubMed link indicates BioLegend citation)
- Gao X, et al. 2015. J Immunol. 194:438. PubMed
- Product Citations
AB_2561333 (BioLegend Cat. No. 516501)
- High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.
- IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
- IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
- Cell Type
- Epithelial cells, Fibroblasts
- Biology Area
- Molecular Family
- Antigen References
1. Schmitz J, et al. Immunity 2005 23:479
2. Barksby HE, et al. C Exp Immunol 2007 149:217
3. Arend WP, et al. ImmRev 2008 223:20
4. Suzukawa M, et al. J. Immunol. 2008 181:5981
5. Moussio C, et al. PlosOne 2008 10:e3331.
- Gene ID
- 77125 View all products for this Gene ID
- View information about IL-33 on UniProt.org
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