- A17013A (See other available formats)
- Other Names
- Leukocyte C-terminal Src kinase, Protein YT16, p56-LCK, T cell-specific protein-tyrosine kinase
- Mouse IgG1, κ
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- Product Citations
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The Src family tyrosine kinase p56Lck (LCK) is a non-receptor tyrosine kinase that is primarily expressed in T cells and NK cells. It plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. LCK, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complex results in the recruitment of CD4- and CD8-bound LCK to the TCR/CD3 signaling complex. LCK then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by LCK. LCK also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of LCK by CSK at Tyrosine 505 negatively regulates LCK, and is proposed to generate a closed, inactive conformation of the protein.Product Details
- Antibody Type
- Host Species
- Human LCK peptide phosphorylated at Tyr 505.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- 509 and 539 amino acid isoforms with predicted molecular weights of 58 and 61 kD, respectively. Contains N-terminal domain with myristylation and palmitoylation sites, a kinase domain, and SH2 and SH3 domains that mediate protein-protein interactions between LCK and other proteins.
- T cell receptor signaling
- AXL, CD2, CD4, CD5, CD8, CD44, CD45, CD122, PI4K, VAV1, RASA1, FYB, CDK1, RAF1, ZAP70, SYK, RUNX3
- Cell Type
- T cells
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
- Phillipsen L, et al. 2017. Sci Signal. 10:eaaf4736.
- Moogk D, et al. 2016. J Immunol. 197:644.
- Klammt C, et al. 2015. Nat Immunol. 16:961.
- Cancer Genome Atlas Network. 2015. Cell. 161:1681.
- Casas J, et al. 2014. Nat Commun. 5:5624
- McNeill L, et al. 2007. Immunnity. 27:425.
- Lefebvre DC, et al. 2003. Biochim Biophys Acta. 1650:40.
- Kabouridis PS. 2003. Biochem J. 371:907.
- Gene ID
- 3932 View all products for this Gene ID
- View information about LCK Phospho on UniProt.org
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