Purified anti-HYOU1 Antibody

Pricing & Availability
Clone
W18144A (See other available formats)
Other Names
Hypoxia Up-Regulated 1, GRP-170, ORP-150, 170 kD Glucose-Regulated Protein 150 kD Oxygen-Regulated Protein
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18144A_PURE_HYOU1_Antibody_1_081519_updated.png
Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-HYOU1 antibody, clone W18144A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18144A_PURE_HYOU1_Antibody_1_081519_updated.png
    Whole cell extracts (15 µg) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-HYOU1 antibody, clone W18144A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP-conjugated goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W18144A_PURE_HYOU1_Antibody_2_081519
    Whole cell lysates (250 µg) were prepared from MOLT-4 cells and then immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or purified HYOU1 antibody (clone W18144A). The resulting IP fractions and 5% whole cell lysate input were resolved by 4-12% Bis-Tris gel electrophoresis and probed with HRP-conjugated goat anti-rat IgG antibody.
  • W18144A_PURE_HYOU1_Antibody_3_081519
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either purified rat IgG2a, κ isotype control antibody (Cat. No. 400502, Panel A) or purified anti-HYOU1 antibody, clone W18144A (Panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
671401 25 µg £57
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671402 100 µg £144
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Description

HYOU1 belongs to the heat shock protein 70 family. The protein encoded by this gene is involved in stress-dependent induction. As a result, under low-oxygen conditions, HYOU1 accumulates in the endoplasmic reticulum. HYOU1 also acts as a nucleotide exchange factor (NEF) that recognizes other proteins within the ER and participates in protein folding.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human HYOU1 protein corresponding to amino acids 750-999.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg per ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When stained for ICC, methanol permeabilization proved more effective than Triton-X 100 permeabilization in localizing expression to the endoplasmic reticulum.

RRID
AB_2814532 (BioLegend Cat. No. 671401)
AB_2814533 (BioLegend Cat. No. 671402)

Antigen Details

Structure
HYOU1 is a 999 amino-acid protein with a predicted molecular weight of 110 kD.
Distribution

Localized to the ER; Ubiquitously expressed

Function
Protein folding & secretion; stress-dependent induction
Molecular Family
Heat Shock Proteins, Organelle Markers
Antigen References
  1. Ozawa K, et al. 1999. J. Biol. Chem. 274(10):6397-404.
  2. Ikeda J, et al. 1997. Biochem. Biophys. Res. Commun. 230(1):94-9.
  3. Takeuchi S. 2006. Protein J. 25(7-8):517-28.
  4. Kaneda S, et al. 2000. J Biochem. 128(3):529-38.
Gene ID
10525 View all products for this Gene ID
UniProt
View information about HYOU1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/15/2019

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