Purified anti-human IL-2 Antibody

Pricing & Availability
Clone
W19046A (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF); Eosinophil differentiation factor (EDF); Killer cell helper factor (KHF); Macrophage-activating factor for cytotoxicity I (MAF-C I); Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2b, κ
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Product Citations
publications
W19046A_PURE_IL-2_Antibody_091120.png
Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) for 6 hours, surface stained with anti-human CD3 FITC, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set. Cells were then stained with purified anti-human IL-2 (clone W19046A) (left) or rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
  • W19046A_PURE_IL-2_Antibody_091120.png
    Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) for 6 hours, surface stained with anti-human CD3 FITC, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set. Cells were then stained with purified anti-human IL-2 (clone W19046A) (left) or rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE.
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310602 100 µg £82.00
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2876610 (BioLegend Cat. No. 310602)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Distribution

T cells

Function
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Ligand/Receptor
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/11/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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