- Poly5166 (See other available formats)
- Other Names
- IL-17, IL-17AF, IL-17A/F, Interleukin 17F, ML-1, ML1, Interleukin 17AF
- Goat Polyclonal Ig
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- Product Citations
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IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions and in a variety of auto-immune diseases.
The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F. It is a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.
BioLegend's purified Poly5166 antibody is IL-17F specific and can be paired with biotinylated Poly5166 to recognize IL-17F or with biotinylated anti-human IL-17A clone BL23 to recognize the IL-17A/F heterodimer.
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.2 - 2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 10.94 to 700 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The pure Poly5166 (Cat. No. 516601) antibody is useful as the capture antibody in a sandwich ELISA assay. Poly5166 can be used as a poly-poly pair with biotinylated Poly5166 to recognize human IL-17F, or it can be used as a poly-mono pair with biotinylated BL23 (Cat. No. 512705) to recognize the human IL-17A/F heterodimer (Cat. No. 580609).
AB_2783337 (BioLegend Cat. No. 516601)
T cells, Th17 cells, memory CD4 T cells, gamma delta T cells, and epithelial cells, activated human mast cells, and basophils
- IL-17F induces various cytokines, chemokines, and adhesion molecules, including IL-6, IL-8, CXCL1, CXCL5, transforming growth factor-b (TGF-b), CCL2, G-CSF, granulocyte macrophage-colony forming factor (GM-CSF) and intracellular adhesion molecule-1 (ICAM-1), in airway epithelial cells, vein endothelial cells, and fibroblasts.
- IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
- Ligand Receptor
- IL-17F utilizes IL-17RA and IL-17RC
- Cell Type
- Basophils, Epithelial cells, Mast cells, T cells, Th17
- Antigen References
- Starnes T, et al. 2001. J. Immunol. 167:4137.
- Kawaguchi M, et al. 2001. J. Immunol. 167:4430.
- Hizawa N, et al. 2006. Clin Experimental Allergy 36:1109.
- Seiderer J, et al. 2008. Inflamm Bowel Dis. 14:437.
- Wu X, et al. 2010 Int J Cancer 127:86.
- Gene ID
- 112744 View all products for this Gene ID
- View information about IL-17F on UniProt.org
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