Purified anti-human IL-17F Antibody

Pricing & Availability
Clone
Poly5166 (See other available formats)
Other Names
IL-17, IL-17AF, IL-17A/F, Interleukin 17F, ML-1, ML1, Interleukin 17AF
Isotype
Goat Polyclonal Ig
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516601 100 µg £151
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Description

IL-17F is part of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. It is expressed by Th17 cells, along with IL-17A homodimer and IL-17A/F heterodimer. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-β, and MCP-1, playing a critical role in the regulation of inflammatory reactions and in a variety of auto-immune diseases.

The IL-17A/F heterodimer consists of the monomeric units of IL-17A and IL-17F. It is a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.

BioLegend's purified Poly5166 antibody is IL-17F specific and can be paired with biotinylated Poly5166 to recognize IL-17F or with biotinylated anti-human IL-17A clone BL23 to recognize the IL-17A/F heterodimer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.2 - 2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-17A/F recombinant protein ranging from 10.94 to 700 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The pure Poly5166 (Cat. No. 516601) antibody is useful as the capture antibody in a sandwich ELISA assay.  Poly5166 can be used as a poly-poly pair with biotinylated Poly5166 to recognize human IL-17F, or it can be used as a poly-mono pair with biotinylated BL23 (Cat. No. 512705) to recognize the human IL-17A/F heterodimer (Cat. No. 580609).

RRID
AB_2783337 (BioLegend Cat. No. 516601)

Antigen Details

Structure
Homodimer
Distribution

T cells, Th17 cells, memory CD4 T cells, gamma delta T cells, and epithelial cells, activated human mast cells, and basophils

Function
IL-17F induces various cytokines, chemokines, and adhesion molecules, including IL-6, IL-8, CXCL1, CXCL5, transforming growth factor-b (TGF-b), CCL2, G-CSF, granulocyte macrophage-colony forming factor (GM-CSF) and intracellular adhesion molecule-1 (ICAM-1), in airway epithelial cells, vein endothelial cells, and fibroblasts.
Interaction
IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
Ligand Receptor
IL-17F utilizes IL-17RA and IL-17RC
Cell Type
Basophils, Epithelial cells, Mast cells, T cells, Th17
Antigen References
  1. Starnes T, et al. 2001. J. Immunol. 167:4137.
  2. Kawaguchi M, et al. 2001. J. Immunol. 167:4430.
  3. Hizawa N, et al. 2006. Clin Experimental Allergy 36:1109.
  4. Seiderer J, et al. 2008. Inflamm Bowel Dis. 14:437.
  5. Wu X, et al. 2010 Int J Cancer 127:86.
Gene ID
112744 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/28/2018

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