Purified anti-human EOS Antibody

Pricing & Availability
Clone
W16032B (See other available formats)
Other Names
Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16032B_PURE_EOS_Antibody_082119_updated
Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with purified EOS (clone W16032B, left) or rat IgG2a, κ isotype (right), and FOXP3 (clone 206D) Alexa Fluor® 647 followed by anti-rat IgG PerCP/Cyanine5.5. Cells shown gated on CD3+CD4+ cells.
  • W16032B_PURE_EOS_Antibody_082119_updated
    Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with purified EOS (clone W16032B, left) or rat IgG2a, κ isotype (right), and FOXP3 (clone 206D) Alexa Fluor® 647 followed by anti-rat IgG PerCP/Cyanine5.5. Cells shown gated on CD3+CD4+ cells.
Cat # Size Price Quantity Avail. Save
397402 100 µg £165
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Description

EOS also known as IKZF4 is an IKAROS Family Member expressed in lymphocytes, specifically in Tregs. It plays an important role in the inhibitory function of Tregs. It may be involved in the development of the central and peripheral nervous systems as well as lymphoid development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EOS recombinant protein (271-520 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814371 (BioLegend Cat. No. 397402)

Antigen Details

Structure
IKAROS family member
Distribution

Lymphocytes, specifically in Tregs

Function
Essential for inhibitory function of Tregs
Interaction
Binds to 5'GGGAATRCC-3' Ikaros-binding sequence
Antigen References
  1. Honma Y, et al. 1999. FEBS Lett. 447:76-80. 
  2. Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54. 
  3. Westman BJ, et al. 2004. Biochemistry. 43:13318-27. 
  4. Pan F, et al. 2009. Science. 10.1126/science.1176077. 
Gene ID
64375 View all products for this Gene ID
UniProt
View information about EOS on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/15/2019

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